LOCUS       BBa_K620002              637 bp    DNA     linear       01-Jun-2024
DEFINITION  LacI promoter + BisdA
ACCESSION   BBa_K620002
VERSION     BBa_K620002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    205..208
                     /label=conserved
     RBS             201..212
                     /label=BBa_B0034
     CDS             213..542
                     /label=BBa_K123000
     stem_loop       550..569
                     /label=T7 TE
     misc_feature    576..576
                     /label=stop
     polyA_site      570..583
                     /label=polya
     misc_feature    543..583
                     /label=BBa_B0012
     terminator      543..583
                     /label=BBa_B0012
     stem_loop       604..626
                     /label=stem_loop
     misc_feature    592..637
                     /label=BBa_B0011
     terminator      592..637
                     /label=BBa_B0011
     terminator      543..637
                     /label=BBa_B0014
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca aaagaggaga aaatggccgg ccctcatatc caagtgacta
      241 cccgcgatgg ggagatacgc gaactcgacg tcgcagcctc cgggttcctg atggaagccc
      301 ttcgcgacgc caatatcgac ggcgtcgagg cgatgtgcgg cggatgctgc tcctgcgcga
      361 cctgccacgt ctacatcgac gctgctcccg ccgggaccct gccgccggtc tcctccgacg
      421 aggagatgct gctttccggc ctggtctcga cccccggacg gtcgcggctc tcctgccaaa
      481 ttccggtcac ggccgaactg gatggcctta agctcacgat cccgccggac tccaccggtt
      541 aatcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat
      601 aaaaagccag attattaatc cggctttttt attattt
//