LOCUS       BBa_K624001             1571 bp    DNA     linear       17-May-2024
DEFINITION  tetR+RBS+GFP+ter
ACCESSION   BBa_K624001
VERSION     BBa_K624001.1  
FEATURES             Location/Qualifiers
     CDS             4..620
                     /label=tetR
     misc_feature    621..654
                     /label=SsrA
     misc_feature    661..685
                     /label=Help:Barcodes
     CDS             1..685
                     /label=BBa_C0040
     RBS             694..708
                     /label=RBS-1\Strong
     RBS             701..705
                     /label=RBS
     misc_feature    694..708
                     /label=BBa_B0030
     RBS             694..708
                     /label=BBa_B0030
     CDS             715..1434
                     /label=GFP protein
     CDS             715..1434
                     /label=BBa_E0040
     stem_loop       1454..1497
                     /label=stem_loop
     misc_feature    1443..1522
                     /label=BBa_B0010
     terminator      1443..1522
                     /label=BBa_B0010
     stem_loop       1538..1557
                     /label=T7 TE
     misc_feature    1564..1564
                     /label=stop
     polyA_site      1558..1571
                     /label=polya
     misc_feature    1531..1571
                     /label=BBa_B0012
     terminator      1531..1571
                     /label=BBa_B0012
     misc_feature    694..1571
                     /label=BBa_E0840
ORIGIN
        1 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
       61 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
      121 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
      181 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
      241 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
      301 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
      361 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
      421 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
      481 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
      541 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
      601 cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa
      661 cactgatagt gctagtgtag atcactacta gagattaaag aggagaaata ctagatgcgt
      721 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      781 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      841 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      901 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      961 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1021 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1081 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1141 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1201 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1261 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1321 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1381 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1441 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1501 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1561 ctgcgtttat a
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