LOCUS BBa_K624019 3770 bp DNA linear 02-May-2024 DEFINITION pYMB essentials+[13-R]+[Y-CHAMP] ACCESSION BBa_K624019 VERSION BBa_K624019.1 FEATURES Location/Qualifiers misc_feature 1..80 /label=ori (pMGT) promoter 81..274 /label=Pmsp1 misc_feature 275..277 /label=start promoter 1..277 /label=BBa_K624000 CDS 313..1142 /label=rep (pMGT) RBS 286..309 /label=rbs from pMGT misc_feature 310..312 /label=start CDS 286..1142 /label=BBa_K624003 CDS 1..1142 /label=BBa_K624004 RBS 1151..1178 /label=rbs (from Pmsp3) misc_feature 1179..1181 /label=artificial start codon RBS 1151..1181 /label=BBa_K624012 CDS 1191..1559 /label=mms13 misc_feature 1188..1190 /label=start CDS 1560..2945 /label=rLuc misc_feature 2946..2948 /label=stop CDS 1188..2948 /label=BBa_K624007 RBS 2957..2984 /label=rbs (from Pmsp3) misc_feature 2985..2987 /label=artificial start codon RBS 2957..2987 /label=BBa_K624012 CDS 2997..3710 /label=EYFP misc_feature 2994..2996 /label=start CDS 3711..3767 /label=CHAMP (anti-H1[mms13]) misc_feature 3768..3770 /label=stop CDS 2994..3770 /label=BBa_K624009 ORIGIN 1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa 61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc 121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg 181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg 241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg 301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt 361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat 421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat 481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg 541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc 601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg 661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac 721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc 781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt 841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc 901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg 961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat 1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc 1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc 1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg cccgaaggct 1201 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 1261 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 1321 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 1381 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 1441 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 1501 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca 1561 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 1621 gcatggacga gctgtacaag atgccctttc accttgcccc ctatctggcg aaatccgttc 1681 ccggcgtcgg cgttctcggc gccctggtcg gcggcgccgc cgccttggcc aagaacgtcc 1741 gcctcctgaa ggaaaagcgc atcaccaata ccgaagcggc catcgatacc ggcaaggaaa 1801 ccgtcggcgc cggcctggcc accgcgcttt ccgccgtggc cgcgaccgcc gtcggcggcg 1861 gcctggttgt atcgctgggc accgccttgg tggccggcgt tgccgccaaa tatgcctggg 1921 atcgcggcgt cgatctggtc gagaaggaac tgaaccgcgg caaagctgcc aacggcgctt 1981 ccgacgagga catcttgcgg gacgaactgg ccatggcttc caaggtgtac gaccccgagc 2041 aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc 2101 tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc gtgatttttc 2161 tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac atcgagcccg 2221 tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag agcgggaatg 2281 gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag ctgctgaacc 2341 ttccaaagaa aatcatcttt gtgggccacg actggggggc ttgtctggcc tttcactact 2401 cctacgagca ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga 2461 tcgagtcctg ggacgagtgg cctgacatcg aggaggatat cgccctgatc aagagcgaag 2521 agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccatgctc ccaagcaaga 2581 tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc aaggagaagg 2641 gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt aagggaggca 2701 agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc agcgacgatc 2761 tgcctaagat gttcatcgag tccgaccctg ggttcttttc caacgctatt gtcgagggag 2821 ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc agccaggagg 2881 acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg 2941 agcagtaata ctagaggtcc aagccctatg agggagcctt tctaatgtac tagatggtga 3001 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 3061 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 3121 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 3181 ccaccttcgg ctacggcctg caatgcttcg cccgctaccc cgaccacatg aagctgcacg 3241 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 3301 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 3361 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 3421 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 3481 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 3541 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 3601 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 3661 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag atgaagaagc 3721 tgttcgtgct gctgatgttc atcatcgtgg ccctgctgtg gaagaagtaa //