LOCUS       BBa_K624021             1907 bp    DNA     linear       29-May-2024
DEFINITION  pYMBG (rbs: Pmsp3) essentials
ACCESSION   BBa_K624021
VERSION     BBa_K624021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     RBS             1151..1178
                     /label=rbs (from Pmsp3)
     misc_feature    1179..1181
                     /label=artificial start codon
     RBS             1151..1181
                     /label=BBa_K624012
     CDS             1188..1907
                     /label=GFP protein
     CDS             1188..1907
                     /label=BBa_E0040
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagag gtccaagccc tatgagggag cctttctaat gtactagatg cgtaaaggag
     1201 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     1261 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     1321 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
     1381 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
     1441 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     1501 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     1561 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     1621 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     1681 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1741 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1801 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1861 cagctgctgg gattacacat ggcatggatg aactatacaa ataataa
//