LOCUS       BBa_K624023             3173 bp    DNA     linear       29-Apr-2024
DEFINITION  pYMB essentials+[Y-13-R]
ACCESSION   BBa_K624023
VERSION     BBa_K624023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /label=ori (pMGT)
     promoter        81..274
                     /label=Pmsp1
     misc_feature    275..277
                     /label=start
     promoter        1..277
                     /label=BBa_K624000
     CDS             313..1142
                     /label=rep (pMGT)
     RBS             286..309
                     /label=rbs from pMGT
     misc_feature    310..312
                     /label=start
     CDS             286..1142
                     /label=BBa_K624003
     CDS             1..1142
                     /label=BBa_K624004
     misc_feature    1149..1151
                     /label=start
     CDS             1152..1865
                     /label=EYFP
     CDS             1866..2237
                     /label=mms13
     CDS             2238..3170
                     /label=rLuc
     misc_feature    3171..3173
                     /label=stop
     CDS             1149..3173
                     /label=BBa_K624006
ORIGIN
        1 tgatccgcca gcgccgaagc catcataact gcattgaata attcaaagtg cattgaataa
       61 ggcagggtgc ataaggcgca gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc
      121 atccatgcca cagtgcggag ttttcgatac agccgacgtc ccgcctcgct tgactggcgg
      181 cgcccatcat ggcgtgatgg cgaccgttcg ttcatttgga tagcggtcgc ggccattggg
      241 ggtcgttttc gcaaagccaa agagaggaga taccatgtac tagagcggga gagggagctg
      301 atcccgctga tggtgacgct gacaggccat cacaatgcac ggatgagcct cgctgaatgt
      361 ctgacggtga tgaagaaggc caagcagagc cttcaccagt ctggcccatg gaagcgccat
      421 gttggcccgg tggtcgaagg gcacgtcacg gcaacggagg tcaatcggtc acgccgccat
      481 ggctggcacg tccattttca cgtcctgatg ctgattgagg ccgccagcga ggccgaagcg
      541 attgcgctcg tgacccgaca cctcgaacct gaatggctcc ggcagcttga gcgccaaggc
      601 agctggggcg gcgaacatgc gttagacgtc cagggcgcag ccgatgcgga tcggtatgtg
      661 gccaagtggg gcgcggctga ggaattggct ctgtcgggcg aaaagcgggc gcgcggcgac
      721 gccgaaatta agggtcaaac gccgtgggaa ttgttggccg ctgcggctga tggtgacgtc
      781 caggctggcc gcgattttgt ggagtacgcg accctgtttc atggcaaacg ccagctcgtt
      841 tggtcgcgag gactgaaaga gcgtgtcggc ctcaaggtgg tggaagacga cgaggccgcc
      901 ggcgagctgg aggatgtcgc agatagcgcc atcaacgaag tggccgtgtt gccgaggccg
      961 acttggcgtc acatcgtccg gcgcaggctc cgcgcggatc tgctggccgc cgctcgtgat
     1021 gacggattcg atggcgtcgc gcggctgctc aatgagcatg gcatcggcgg agctatgccc
     1081 ggcggctggc gaccaatgct tagcgaatat accgagcata ttgcgatggc tgcgtgactc
     1141 tttactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1201 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1261 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1321 ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc
     1381 acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1441 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1501 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1561 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1621 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1681 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1741 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1801 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1861 acaagatgcc ctttcacctt gccccctatc tggcgaaatc cgttcccggc gtcggcgttc
     1921 tcggcgccct ggtcggcggc gccgccgcct tggccaagaa cgtccgcctc ctgaaggaaa
     1981 agcgcatcac caataccgaa gcggccatcg ataccggcaa ggaaaccgtc ggcgccggcc
     2041 tggccaccgc gctttccgcc gtggccgcga ccgccgtcgg cggcggcctg gttgtatcgc
     2101 tgggcaccgc cttggtggcc ggcgttgccg ccaaatatgc ctgggatcgc ggcgtcgatc
     2161 tggtcgagaa ggaactgaac cgcggcaaag ctgccaacgg cgcttccgac gaggacatct
     2221 tgcgggacga actggccatg gcttccaagg tgtacgaccc cgagcaacgc aaacgcatga
     2281 tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac tccttcatca
     2341 actactatga ttccgagaag cacgccgaga acgccgtgat ttttctgcat ggtaacgctg
     2401 cctccagcta cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct agatgcatca
     2461 tccctgatct gatcggaatg ggtaagtccg gcaagagcgg gaatggctca tatcgcctcc
     2521 tggatcacta caagtacctc accgcttggt tcgagctgct gaaccttcca aagaaaatca
     2581 tctttgtggg ccacgactgg ggggcttgtc tggcctttca ctactcctac gagcaccaag
     2641 acaagatcaa ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag tcctgggacg
     2701 agtggcctga catcgaggag gatatcgccc tgatcaagag cgaagagggc gagaaaatgg
     2761 tgcttgagaa taacttcttc gtcgagacca tgctcccaag caagatcatg cggaaactgg
     2821 agcctgagga gttcgctgcc tacctggagc cattcaagga gaagggcgag gttagacggc
     2881 ctaccctctc ctggcctcgc gagatccctc tcgttaaggg aggcaagccc gacgtcgtcc
     2941 agattgtccg caactacaac gcctaccttc gggccagcga cgatctgcct aagatgttca
     3001 tcgagtccga ccctgggttc ttttccaacg ctattgtcga gggagctaag aagttcccta
     3061 acaccgagtt cgtgaaggtg aagggcctcc acttcagcca ggaggacgct ccagatgaaa
     3121 tgggtaagta catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag taa
//