LOCUS       BBa_K624037             1437 bp    DNA     linear       12-May-2024
DEFINITION  rbs+minC+mcherry
ACCESSION   BBa_K624037
VERSION     BBa_K624037.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..717
                     /label=minC
     primer_bind     697..717
                     /label=reverse minC primer binding
     primer_bind     19..46
                     /label=forward minC primer binding
     CDS             19..717
                     /label=BBa_K624033
     CDS             724..1434
                     /label=mCherry
     misc_feature    1075..1075
                     /label=C->T (removing PstI site)
     misc_feature    724..1437
                     /label=BBa_J06504
ORIGIN
        1 aaagaggaga aatactagat gtcaaacacg ccaatcgagc ttaaaggcag tagcttcact
       61 ttatctgtgg ttcatctgca tgaggcagaa cctaaggtta tccatcaggc gctggaagac
      121 aaaatcgctc aggcccccgc atttttaaaa catgcccccg ttgtactcaa cgtcagtgca
      181 ctggaagacc cggtaaactg gtcagcgatg cataaggcgg tttcggcaac cggtttgcgg
      241 gttattggcg taagtggctg caaagatgcg caacttaaag ccgaaattga aaagatgggg
      301 ctgcctatcc tgacggaagg aaaggaaaaa gcgccacgtc cagctcccac accgcaggct
      361 ccagcgcaaa atacaacgcc ggtcacaaaa acgcgtttaa tagatacccc ggtgcgttcc
      421 ggtcagcgta tttatgctcc acaatgtgat ctgattgtta caagccacgt tagcgctggg
      481 gccgaattga ttgccgatgg gaacattcat gtctatggca tgatgcgcgg tcgtgcgctg
      541 gcaggggcaa gtggtgaccg ggaaacgcaa atattttgta cgaacctgat ggcggaactg
      601 gtgtccatcg caggtgaata ctggctgagt gatcaaatcc cagcagaatt ttatggcaaa
      661 gcggcgcgac tgcaattagt cgaaaacgct ttgaccgttc aaccgttaaa ttaataatac
      721 tagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      781 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      841 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      901 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      961 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     1021 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccttgcag
     1081 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     1141 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     1201 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     1261 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     1321 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     1381 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaataa
//