LOCUS       BBa_K624045             1701 bp    DNA     linear       26-May-2024
DEFINITION  Pmsp1(tetO) + RBS(Pmsp3) + minC + RBS(Pmsp3) + mcherry
ACCESSION   BBa_K624045
VERSION     BBa_K624045.1  
FEATURES             Location/Qualifiers
     promoter        1..198
                     /label=Pmsp1[tetO]
     misc_feature    90..95
                     /label=-35 box
     misc_feature    96..110
                     /label=tetO
     misc_feature    111..119
                     /label=-10 box
     promoter        1..198
                     /label=BBa_K624046
     RBS             207..234
                     /label=rbs (from Pmsp3)
     misc_feature    235..237
                     /label=artificial start codon
     RBS             207..237
                     /label=BBa_K624012
     CDS             244..942
                     /label=minC
     primer_bind     922..942
                     /label=reverse minC primer binding
     primer_bind     244..271
                     /label=forward minC primer binding
     CDS             244..942
                     /label=BBa_K624033
     RBS             951..978
                     /label=rbs (from Pmsp3)
     misc_feature    979..981
                     /label=artificial start codon
     RBS             951..981
                     /label=BBa_K624012
     CDS             988..1698
                     /label=mCherry
     misc_feature    1339..1339
                     /label=C->T (removing PstI site)
     misc_feature    988..1701
                     /label=BBa_J06504
ORIGIN
        1 gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc atccatgcca cagtgcggag
       61 ttttcgatac agccgacgtc ccgcctcgct tgactcctat cagtgataga tggcgtgatg
      121 gcgaccgttc gttcatttgg atagcggtcg cggccattgg gggtcgtttt cgcaaagcca
      181 aagagaggag ataccatgta ctagaggtcc aagccctatg agggagcctt tctaatgtac
      241 tagatgtcaa acacgccaat cgagcttaaa ggcagtagct tcactttatc tgtggttcat
      301 ctgcatgagg cagaacctaa ggttatccat caggcgctgg aagacaaaat cgctcaggcc
      361 cccgcatttt taaaacatgc ccccgttgta ctcaacgtca gtgcactgga agacccggta
      421 aactggtcag cgatgcataa ggcggtttcg gcaaccggtt tgcgggttat tggcgtaagt
      481 ggctgcaaag atgcgcaact taaagccgaa attgaaaaga tggggctgcc tatcctgacg
      541 gaaggaaagg aaaaagcgcc acgtccagct cccacaccgc aggctccagc gcaaaataca
      601 acgccggtca caaaaacgcg tttaatagat accccggtgc gttccggtca gcgtatttat
      661 gctccacaat gtgatctgat tgttacaagc cacgttagcg ctggggccga attgattgcc
      721 gatgggaaca ttcatgtcta tggcatgatg cgcggtcgtg cgctggcagg ggcaagtggt
      781 gaccgggaaa cgcaaatatt ttgtacgaac ctgatggcgg aactggtgtc catcgcaggt
      841 gaatactggc tgagtgatca aatcccagca gaattttatg gcaaagcggc gcgactgcaa
      901 ttagtcgaaa acgctttgac cgttcaaccg ttaaattaat aatactagag gtccaagccc
      961 tatgagggag cctttctaat gtactagatg gtgagcaagg gcgaggagga taacatggcc
     1021 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     1081 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     1141 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     1201 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     1261 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     1321 gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     1381 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     1441 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     1501 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     1561 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     1621 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     1681 gacgagctgt acaagtaata a
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