LOCUS       BBa_K624049             1495 bp    DNA     linear       10-May-2024
DEFINITION  RBS(Pmsp3) + minC + RBS(Pmsp3) +mcherry
ACCESSION   BBa_K624049
VERSION     BBa_K624049.1  
FEATURES             Location/Qualifiers
     RBS             1..28
                     /label=rbs (from Pmsp3)
     misc_feature    29..31
                     /label=artificial start codon
     RBS             1..31
                     /label=BBa_K624012
     CDS             38..736
                     /label=minC
     primer_bind     716..736
                     /label=reverse minC primer binding
     primer_bind     38..65
                     /label=forward minC primer binding
     CDS             38..736
                     /label=BBa_K624033
     RBS             745..772
                     /label=rbs (from Pmsp3)
     misc_feature    773..775
                     /label=artificial start codon
     RBS             745..775
                     /label=BBa_K624012
     CDS             782..1492
                     /label=mCherry
     misc_feature    1133..1133
                     /label=C->T (removing PstI site)
     misc_feature    782..1495
                     /label=BBa_J06504
ORIGIN
        1 gtccaagccc tatgagggag cctttctaat gtactagatg tcaaacacgc caatcgagct
       61 taaaggcagt agcttcactt tatctgtggt tcatctgcat gaggcagaac ctaaggttat
      121 ccatcaggcg ctggaagaca aaatcgctca ggcccccgca tttttaaaac atgcccccgt
      181 tgtactcaac gtcagtgcac tggaagaccc ggtaaactgg tcagcgatgc ataaggcggt
      241 ttcggcaacc ggtttgcggg ttattggcgt aagtggctgc aaagatgcgc aacttaaagc
      301 cgaaattgaa aagatggggc tgcctatcct gacggaagga aaggaaaaag cgccacgtcc
      361 agctcccaca ccgcaggctc cagcgcaaaa tacaacgccg gtcacaaaaa cgcgtttaat
      421 agataccccg gtgcgttccg gtcagcgtat ttatgctcca caatgtgatc tgattgttac
      481 aagccacgtt agcgctgggg ccgaattgat tgccgatggg aacattcatg tctatggcat
      541 gatgcgcggt cgtgcgctgg caggggcaag tggtgaccgg gaaacgcaaa tattttgtac
      601 gaacctgatg gcggaactgg tgtccatcgc aggtgaatac tggctgagtg atcaaatccc
      661 agcagaattt tatggcaaag cggcgcgact gcaattagtc gaaaacgctt tgaccgttca
      721 accgttaaat taataatact agaggtccaa gccctatgag ggagcctttc taatgtacta
      781 gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
      841 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      901 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
      961 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1021 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     1081 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccttgcagga
     1141 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1201 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     1261 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     1321 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     1381 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     1441 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aataa
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