LOCUS       BBa_K624050             1477 bp    DNA     linear       11-May-2024
DEFINITION  RBS(trunc.) + minC + RBS(trunc.) + mcherry
ACCESSION   BBa_K624050
VERSION     BBa_K624050.1  
FEATURES             Location/Qualifiers
     RBS             1..22
                     /label=rbs (trunc.) from Pmsp3
     RBS             1..22
                     /label=BBa_K624013
     CDS             29..727
                     /label=minC
     primer_bind     707..727
                     /label=reverse minC primer binding
     primer_bind     29..56
                     /label=forward minC primer binding
     CDS             29..727
                     /label=BBa_K624033
     RBS             736..757
                     /label=rbs (trunc.) from Pmsp3
     RBS             736..757
                     /label=BBa_K624013
     CDS             764..1474
                     /label=mCherry
     misc_feature    1115..1115
                     /label=C->T (removing PstI site)
     misc_feature    764..1477
                     /label=BBa_J06504
ORIGIN
        1 gtccaagccc tatgagggag cctactagat gtcaaacacg ccaatcgagc ttaaaggcag
       61 tagcttcact ttatctgtgg ttcatctgca tgaggcagaa cctaaggtta tccatcaggc
      121 gctggaagac aaaatcgctc aggcccccgc atttttaaaa catgcccccg ttgtactcaa
      181 cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg cataaggcgg tttcggcaac
      241 cggtttgcgg gttattggcg taagtggctg caaagatgcg caacttaaag ccgaaattga
      301 aaagatgggg ctgcctatcc tgacggaagg aaaggaaaaa gcgccacgtc cagctcccac
      361 accgcaggct ccagcgcaaa atacaacgcc ggtcacaaaa acgcgtttaa tagatacccc
      421 ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat ctgattgtta caagccacgt
      481 tagcgctggg gccgaattga ttgccgatgg gaacattcat gtctatggca tgatgcgcgg
      541 tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa atattttgta cgaacctgat
      601 ggcggaactg gtgtccatcg caggtgaata ctggctgagt gatcaaatcc cagcagaatt
      661 ttatggcaaa gcggcgcgac tgcaattagt cgaaaacgct ttgaccgttc aaccgttaaa
      721 ttaataatac tagaggtcca agccctatga gggagcctac tagatggtga gcaagggcga
      781 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      841 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      901 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      961 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     1021 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     1081 cggcggcgtg gtgaccgtga cccaggactc ctccttgcag gacggcgagt tcatctacaa
     1141 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     1201 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     1261 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     1321 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     1381 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     1441 ctccaccggc ggcatggacg agctgtacaa gtaataa
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