LOCUS       BBa_K624052             1683 bp    DNA     linear       11-May-2024
DEFINITION  Pmsp1(tetO) + RBS(trunc.) + minC + RBS(trunc.) + mcherry
ACCESSION   BBa_K624052
VERSION     BBa_K624052.1  
FEATURES             Location/Qualifiers
     promoter        1..198
                     /label=Pmsp1[tetO]
     misc_feature    90..95
                     /label=-35 box
     misc_feature    96..110
                     /label=tetO
     misc_feature    111..119
                     /label=-10 box
     promoter        1..198
                     /label=BBa_K624046
     RBS             207..228
                     /label=rbs (trunc.) from Pmsp3
     RBS             207..228
                     /label=BBa_K624013
     CDS             235..933
                     /label=minC
     primer_bind     913..933
                     /label=reverse minC primer binding
     primer_bind     235..262
                     /label=forward minC primer binding
     CDS             235..933
                     /label=BBa_K624033
     RBS             942..963
                     /label=rbs (trunc.) from Pmsp3
     RBS             942..963
                     /label=BBa_K624013
     CDS             970..1680
                     /label=mCherry
     misc_feature    1321..1321
                     /label=C->T (removing PstI site)
     misc_feature    970..1683
                     /label=BBa_J06504
ORIGIN
        1 gcggtaacgc cgcctcgccg gtggaagggt gttgtgtggc atccatgcca cagtgcggag
       61 ttttcgatac agccgacgtc ccgcctcgct tgactcctat cagtgataga tggcgtgatg
      121 gcgaccgttc gttcatttgg atagcggtcg cggccattgg gggtcgtttt cgcaaagcca
      181 aagagaggag ataccatgta ctagaggtcc aagccctatg agggagccta ctagatgtca
      241 aacacgccaa tcgagcttaa aggcagtagc ttcactttat ctgtggttca tctgcatgag
      301 gcagaaccta aggttatcca tcaggcgctg gaagacaaaa tcgctcaggc ccccgcattt
      361 ttaaaacatg cccccgttgt actcaacgtc agtgcactgg aagacccggt aaactggtca
      421 gcgatgcata aggcggtttc ggcaaccggt ttgcgggtta ttggcgtaag tggctgcaaa
      481 gatgcgcaac ttaaagccga aattgaaaag atggggctgc ctatcctgac ggaaggaaag
      541 gaaaaagcgc cacgtccagc tcccacaccg caggctccag cgcaaaatac aacgccggtc
      601 acaaaaacgc gtttaataga taccccggtg cgttccggtc agcgtattta tgctccacaa
      661 tgtgatctga ttgttacaag ccacgttagc gctggggccg aattgattgc cgatgggaac
      721 attcatgtct atggcatgat gcgcggtcgt gcgctggcag gggcaagtgg tgaccgggaa
      781 acgcaaatat tttgtacgaa cctgatggcg gaactggtgt ccatcgcagg tgaatactgg
      841 ctgagtgatc aaatcccagc agaattttat ggcaaagcgg cgcgactgca attagtcgaa
      901 aacgctttga ccgttcaacc gttaaattaa taatactaga ggtccaagcc ctatgaggga
      961 gcctactaga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1021 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1081 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1141 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1201 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1261 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1321 ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1381 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     1441 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1501 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1561 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1621 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     1681 taa
//