BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K634000 1 BBa_K634000 exaD 2011-09-11T11:00:00Z 2015-05-08T01:12:57Z De novo synthesis, codon optimized for E. coli. Original amino acid sequence taken from Pseudomonas aeruginosa. ExaD phosphorylates ExaE, which is a transcription factor acting upon PexaA. false false _809_ 0 9290 9 It's complicated false The synthesis included a Shine-Dalgarno-complimentary RBS 5' of the gene. false Eric Walters annotation2127311 1 exaD range2127311 1 23 841 annotation2127310 1 RBS range2127310 1 9 22 BBa_K634002 1 BBa_K634002 exaDE 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z Cloning of k634000 and k634001. The 2 component system that responds to ethanol that activates transcription of pexaA. false false _809_ 0 9393 9 Not in stock false Each part contains a RBS but the part does not contain a promoter. false Kenneth O. Xu component2129709 1 BBa_K634000 component2129712 1 BBa_K634001 annotation2129709 1 BBa_K634000 range2129709 1 1 841 annotation2129712 1 BBa_K634001 range2129712 1 850 1541 BBa_E0840 1 GFP genera GFP generator 2004-10-17T11:00:00Z 2015-08-31T04:07:26Z Released HQ 2013 B0030.E0040.B0015 false true _11_1_ 0 61 7 In stock true true Jennifer Braff component1249242 1 BBa_E0040 component1249257 1 BBa_B0012 component1249247 1 BBa_B0010 component1249239 1 BBa_B0030 annotation1249257 1 BBa_B0012 range1249257 1 838 878 annotation1249242 1 BBa_E0040 range1249242 1 22 741 annotation1249247 1 BBa_B0010 range1249247 1 750 829 annotation1249239 1 BBa_B0030 range1249239 1 1 15 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K634003 1 BBa_K634003 exaDE-GFP 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z Cloning of BBa_K634002 and E0840. This part is just BBa_K634003 with a GFP (E0840) for verify transcription. false false _809_ 0 9393 9 Not in stock false Contains a double terminator at the 3' end. false Kenneth O. Xu component2129719 1 BBa_K634002 component2129730 1 BBa_E0840 annotation2129730 1 BBa_E0840 range2129730 1 1550 2427 annotation2129719 1 BBa_K634002 range2129719 1 1 1541 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K634001 1 BBa_K634001 exaE 2011-09-15T11:00:00Z 2015-05-08T01:12:57Z de novo synthesis original amino acid sequence from pseudomonas aeruginosa. Released HQ 2013 exaE is a transcription factor that is activated by phosphorylation from exaD. It acts upon pexaA. false false _809_ 0 9393 9 In stock false contains no biobrick cutsites false Kenneth O. Xu annotation2129706 1 exaE range2129706 1 15 692 annotation2129705 1 rbs range2129705 1 1 14 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K634001_sequence 1 aggaggaaataaaaatgaaaattctgctggttgatgatcattttgtggttcgtgaaggtctggcagcactgctgcgtggtctgctgccggatatggaagttaatgaagccggtgatggtgaagaagcactgcaagcagttcagcgtgaaattccgagcctggttattgttgatctgggtctgcctggtattagcggtctggaactgacccgtcgtctgcgtcagcgtctgccgcaactgcgtgttctgttttttagcctgcatgatgaactggcactggttcgtcaggcactggatgccggtgcacgtggttatgttaccaaacgtgcagcaccgaccgttctgctggaagcaattcgtcgtgttctggcaggtcaactgtatctggaacagccgctggcaacccgtctggcatgtcagagctgggaagaacagggtggtgcagcactgcgtggcctgacccgtcgcgaatttgaaatttttcgtctgctggcacgtggtctggcactgcgtgaagttgcagaacatctgggtgttagcgcaaaaaccgttagcaatcatgttagcctgctgaaacagaaactgcaagttagcacccaggcagaactggttcatcgtgccattgatagcggtgttctgcgcctgggactgccgctgccgctggcagaagaagcacgcggtagcctgggttaa BBa_K634003_sequence 1 gtactagaaggaggaaataaaaatggatcgtagccgtcgtcatgcacatcgtcgtcatccgggtgttgcacatccggcagatccgcagcctgcacgtgttcgttttccgctgtttccgcctccgcgtccgggtaaaacctgtcgtctgcgtcgtcgtaccggtctgagtgatcgtccgctgagccatgcaatggatgatctgcgtccggctggtccggcagaatatcgttggctgctggcagttcaggaacgtgaacgtcgtctgctggcacaggcagttcatgatgatattggtcagtatctgaccggtattcgtacccagttactgctgctgcgttgtacagccggtgatggtcagcctgcactggcacgtctggaacgttattgtcagggtctggatgatagctttcgtgcactgctgcgtggtctgcctccgcctgcactgcaacgtcagagcctggatgaagcactgcgtgcatgtgttgcagattggcaggaaggtcagggtattgcctgtgaactgcgtctgggtggtccgctgcctccgctggatgcagcagcaggcacccatctgtatcgtctgctgcaagaagcactgaataacgttgcacgtcatgccggtgcaagccgtgttcgtattcgtctgcaatgtcgtggtggttttctgtatctgagcgttcgtgatgatggtcgtggttgtgcagcagatccggcaccgggttttggtctggatagtatgcgtcagcgtgcacgttgtctgggtgcagaactgagcctgcgtagccgtccgggtcagggcctggcactgcgtctgcgtatgcctctggaacgtgatgattaatactagagaggaggaaataaaaatgaaaattctgctggttgatgatcattttgtggttcgtgaaggtctggcagcactgctgcgtggtctgctgccggatatggaagttaatgaagccggtgatggtgaagaagcactgcaagcagttcagcgtgaaattccgagcctggttattgttgatctgggtctgcctggtattagcggtctggaactgacccgtcgtctgcgtcagcgtctgccgcaactgcgtgttctgttttttagcctgcatgatgaactggcactggttcgtcaggcactggatgccggtgcacgtggttatgttaccaaacgtgcagcaccgaccgttctgctggaagcaattcgtcgtgttctggcaggtcaactgtatctggaacagccgctggcaacccgtctggcatgtcagagctgggaagaacagggtggtgcagcactgcgtggcctgacccgtcgcgaatttgaaatttttcgtctgctggcacgtggtctggcactgcgtgaagttgcagaacatctgggtgttagcgcaaaaaccgttagcaatcatgttagcctgctgaaacagaaactgcaagttagcacccaggcagaactggttcatcgtgccattgatagcggtgttctgcgcctgggactgccgctgccgctggcagaagaagcacgcggtagcctgggttaatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0030_sequence 1 attaaagaggagaaa BBa_K634000_sequence 1 gtactagaaggaggaaataaaaatggatcgtagccgtcgtcatgcacatcgtcgtcatccgggtgttgcacatccggcagatccgcagcctgcacgtgttcgttttccgctgtttccgcctccgcgtccgggtaaaacctgtcgtctgcgtcgtcgtaccggtctgagtgatcgtccgctgagccatgcaatggatgatctgcgtccggctggtccggcagaatatcgttggctgctggcagttcaggaacgtgaacgtcgtctgctggcacaggcagttcatgatgatattggtcagtatctgaccggtattcgtacccagttactgctgctgcgttgtacagccggtgatggtcagcctgcactggcacgtctggaacgttattgtcagggtctggatgatagctttcgtgcactgctgcgtggtctgcctccgcctgcactgcaacgtcagagcctggatgaagcactgcgtgcatgtgttgcagattggcaggaaggtcagggtattgcctgtgaactgcgtctgggtggtccgctgcctccgctggatgcagcagcaggcacccatctgtatcgtctgctgcaagaagcactgaataacgttgcacgtcatgccggtgcaagccgtgttcgtattcgtctgcaatgtcgtggtggttttctgtatctgagcgttcgtgatgatggtcgtggttgtgcagcagatccggcaccgggttttggtctggatagtatgcgtcagcgtgcacgttgtctgggtgcagaactgagcctgcgtagccgtccgggtcagggcctggcactgcgtctgcgtatgcctctggaacgtgatgattaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0840_sequence 1 attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K634002_sequence 1 gtactagaaggaggaaataaaaatggatcgtagccgtcgtcatgcacatcgtcgtcatccgggtgttgcacatccggcagatccgcagcctgcacgtgttcgttttccgctgtttccgcctccgcgtccgggtaaaacctgtcgtctgcgtcgtcgtaccggtctgagtgatcgtccgctgagccatgcaatggatgatctgcgtccggctggtccggcagaatatcgttggctgctggcagttcaggaacgtgaacgtcgtctgctggcacaggcagttcatgatgatattggtcagtatctgaccggtattcgtacccagttactgctgctgcgttgtacagccggtgatggtcagcctgcactggcacgtctggaacgttattgtcagggtctggatgatagctttcgtgcactgctgcgtggtctgcctccgcctgcactgcaacgtcagagcctggatgaagcactgcgtgcatgtgttgcagattggcaggaaggtcagggtattgcctgtgaactgcgtctgggtggtccgctgcctccgctggatgcagcagcaggcacccatctgtatcgtctgctgcaagaagcactgaataacgttgcacgtcatgccggtgcaagccgtgttcgtattcgtctgcaatgtcgtggtggttttctgtatctgagcgttcgtgatgatggtcgtggttgtgcagcagatccggcaccgggttttggtctggatagtatgcgtcagcgtgcacgttgtctgggtgcagaactgagcctgcgtagccgtccgggtcagggcctggcactgcgtctgcgtatgcctctggaacgtgatgattaatactagagaggaggaaataaaaatgaaaattctgctggttgatgatcattttgtggttcgtgaaggtctggcagcactgctgcgtggtctgctgccggatatggaagttaatgaagccggtgatggtgaagaagcactgcaagcagttcagcgtgaaattccgagcctggttattgttgatctgggtctgcctggtattagcggtctggaactgacccgtcgtctgcgtcagcgtctgccgcaactgcgtgttctgttttttagcctgcatgatgaactggcactggttcgtcaggcactggatgccggtgcacgtggttatgttaccaaacgtgcagcaccgaccgttctgctggaagcaattcgtcgtgttctggcaggtcaactgtatctggaacagccgctggcaacccgtctggcatgtcagagctgggaagaacagggtggtgcagcactgcgtggcctgacccgtcgcgaatttgaaatttttcgtctgctggcacgtggtctggcactgcgtgaagttgcagaacatctgggtgttagcgcaaaaaccgttagcaatcatgttagcctgctgaaacagaaactgcaagttagcacccaggcagaactggttcatcgtgccattgatagcggtgttctgcgcctgggactgccgctgccgctggcagaagaagcacgcggtagcctgggttaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z