BBa_K638402

BBa_K638402 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K638402

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Matthew Jones

Date created: 2011-09-18 11:00:00

Date modified: 2015-05-08 01:12:57

TorA tag variant

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_K638402_sequence (Version 1)

Description

We found a TorA sequence which had been successfully used to export GFP to the periplasm of E.coli.
The sequence was originally taken from this paper:
Export of active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli
Joanna D. Thomas1, Richard A. Daniel2, Jeff Errington2, Colin Robinson1,*

The sequence was added into our constructs as part of a gibson assembly primer.

Notes

This part was checked for illeagal restriction sites and none were found.

Source

De novo synthesis based on a sequence from the literature.

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K638402/1

Member of these Collections

• iGEM Parts Registry