BBa_K638403
1
BBa_K638403
Arabinose inducible TorA-Reflectin A1-sfGFP generator
2011-09-19T11:00:00Z
2015-05-08T01:12:57Z
This construct was assembled from its parts using Gibson Assembly.
The reflectin A1 sequence was kindly provided by Dr Wendy Crookes-Goodson.
The TorA tag was synthesised in two overlapping pieces, which were combined by PCR.
This construct is a reflectin A1 protein with a TorA signal peptide on the N terminus, and superfolder GFP (sfGFP) fused at the C-terminus. The reflectin A1 sequence is codon-optimized for E coli. The TorA signal peptide has been shown to allow export of GFP via the twin arginine pathway. The pBAD promoter is inducible with arabinose.
false
false
_814_
0
10177
9
It's complicated
false
NA
false
Joseph Harvey
annotation2135412
1
BBa_K638402
range2135412
1
1237
1365
annotation2135422
1
polya
range2135422
1
3278
3281
annotation2135413
1
reflectin A1
range2135413
1
1366
2424
annotation2135411
1
conserved
range2135411
1
1223
1226
annotation2135408
1
PC
range2135408
1
1043
1114
annotation2135415
1
BBa_I746916
range2135415
1
2425
3144
annotation2135423
1
stop
range2135423
1
3274
3274
annotation2135410
1
BBa_B0034
range2135410
1
1219
1230
annotation2135409
1
Pbad
range2135409
1
1080
1210
annotation2135421
1
T7 TE
range2135421
1
3248
3277
annotation2135416
1
superfolder GFP coding region
range2135416
1
2425
3144
annotation2135406
1
AraC
range2135406
1
1
1079
annotation2135407
1
BBa_I0500
range2135407
1
1
1210
annotation2135414
1
start
range2135414
1
2425
2427
annotation2135420
1
BBa_B0012
range2135420
1
3241
3281
annotation2135417
1
stop
range2135417
1
3139
3144
annotation2135419
1
stem_loop
range2135419
1
3164
3207
annotation2135418
1
BBa_B0010
range2135418
1
3153
3232
BBa_K638403_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggcgaacaacgacttatttcaggcttctcggcgtcgctttctggcgcagctgggcggattaacggtggcgggtatgttgggcccgtcgctgttgactcctcgcagagcgacggcagcacaagccgcaatgggatccatgaaccgttacctgaatcgtcaacgcctgtataacatgtaccgcaataaatatcgtggcgtaatggaaccgatgtcgcgcatgacgatggatttccagggtcgttatatggattcgcagggccgcatggtagacccgcgctactacgactactacgggcgcatgcatgatcatgatcgctattatggccgtagtatgttcaatcagggtcattcaatggatagccagcgttatgggggctggatggacaatccggagcgctatatggacatgagcggttaccagatggatatgcaggggcgttggatggatgcgcagggtcgcttcaacaatccatttggtcaaatgtggcatggtcgtcagggtcattacccggggtacatgagctcacattccatgtacggtcgcaatatgtataacccatatcatagccattatgcgagccgccattttgattcgccagagcgttggatggatatgtctggttaccagatggacatgcaaggtcgttggatggataattacggtcgctatgtaaatccgttcaatcatcacatgtacggtcgcaacatgtgctatccgtatgggaatcactattataaccgtcacatggaacatccggagcgttatatggacatgtccggctaccaaatggacatgcaaggtcgctggatggatacccatggccgccattgcaacccgtttggccagatgtggcataatcgccatggctactatccgggtcatccacatggtcgcaacatgtttcagccggaacgctggatggatatgtctggttatcagatggatatgcaggggcgctggatggataattatggccgttacgtgaatccattttcacataactatgggcgccacatgaactacccgggtggccattataattaccatcacggtcgttacatgaaccacccagaacgtcacatggatatgagctcctaccagatggacatgcatggccgttggatggataatcagggccgctatattgataacttcgatcgtaattattacgattaccacatgtacatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z