BBa_K648013

BBa_K648013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K648013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jim Rose
Date created: 2011-07-03 11:00:00
Date modified: 2016-01-25 02:32:21

GFP with Standard 25 Prefix/Suffix



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K648013_sequence (Version 1)

    Description

    This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.

    Notes

    This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly.
    It was synthesized through PCR oligo synthesis methods using the following primers:

    Forward (56.88 degrees)
    TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC

    Reverse (56.95 degrees)
    AATACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGTCGTCATCATCTTTATAAT

    Source

    This part is similar to the part BBa_E0040 commonly used as a reporter.

    Sequence Annotation Location Component / Role(s)
    misc
    		1,735
    		feature/misc sequence_feature

    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K648013/1
     

    Attachments

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