LOCUS       BBa_K648129             1910 bp    DNA     linear       18-May-2024
DEFINITION  Pr of Or tester with cI repressor with slightly weak RBS
ACCESSION   BBa_K648129
VERSION     BBa_K648129.1  
FEATURES             Location/Qualifiers
     CDS             1..82
                     /label=BBa_K648029
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             109..816
                     /label=mCherry
     misc_feature    460..460
                     /label=C->T (removing PstI site)
     misc_feature    817..825
                     /label=LVA
     misc_feature    109..831
                     /label=BBa_J06505
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
     terminator      840..968
                     /label=BBa_B0015
     misc_feature    91..968
                     /label=BBa_K648000
     promoter        977..1107
                     /label=promoter
     misc_feature    1016..1033
                     /label=AraI1
     misc_feature    1037..1054
                     /label=AraI2
     promoter        977..1106
                     /label=BBa_K206000
     RBS             1115..1129
                     /label=RBS-1\Strong
     RBS             1122..1126
                     /label=RBS
     misc_feature    1115..1129
                     /label=BBa_B0030
     RBS             1115..1129
                     /label=BBa_B0030
     CDS             1139..1846
                     /label=cI lambda
     misc_feature    1847..1879
                     /label=LVA
     misc_feature    1886..1910
                     /label=Help:Barcodes
     CDS             1136..1910
                     /label=BBa_C0051
ORIGIN
        1 acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac
       61 ctctggcggt gataatggtt gctactagag aaagaggaga aatactagat ggtgagcaag
      121 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      181 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      241 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      301 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      361 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      421 gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc
      481 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      541 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      601 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      661 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
      721 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
      781 cgccactcca ccggcggcat ggacgagctg tacaagttag tagcttaata atactagagc
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttatata ctagagacat tgattatttg cacggcgtca cactttgcta tgccatagca
     1021 agatagtcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
     1081 tctccatacc gtttttttgg gctagctact agagattaaa gaggagaaat actagatgag
     1141 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
     1201 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
     1261 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
     1321 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
     1381 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
     1441 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt
     1501 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
     1561 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
     1621 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
     1681 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
     1741 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
     1801 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcgctg caaacgacga
     1861 aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc
//