LOCUS       BBa_K648130             1909 bp    DNA     linear       18-May-2024
DEFINITION  Pr of Or tester with cI repressor with very weak RBS
ACCESSION   BBa_K648130
VERSION     BBa_K648130.1  
FEATURES             Location/Qualifiers
     CDS             1..82
                     /label=BBa_K648029
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             109..816
                     /label=mCherry
     misc_feature    460..460
                     /label=C->T (removing PstI site)
     misc_feature    817..825
                     /label=LVA
     misc_feature    109..831
                     /label=BBa_J06505
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
     terminator      840..968
                     /label=BBa_B0015
     misc_feature    91..968
                     /label=BBa_K648000
     promoter        977..1107
                     /label=promoter
     misc_feature    1016..1033
                     /label=AraI1
     misc_feature    1037..1054
                     /label=AraI2
     promoter        977..1106
                     /label=BBa_K206000
     misc_feature    1121..1124
                     /label=conserved
     RBS             1115..1128
                     /label=BBa_B0031
     CDS             1138..1845
                     /label=cI lambda
     misc_feature    1846..1878
                     /label=LVA
     misc_feature    1885..1909
                     /label=Help:Barcodes
     CDS             1135..1909
                     /label=BBa_C0051
ORIGIN
        1 acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg actattttac
       61 ctctggcggt gataatggtt gctactagag aaagaggaga aatactagat ggtgagcaag
      121 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      181 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      241 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      301 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      361 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      421 gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc
      481 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      541 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      601 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      661 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
      721 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
      781 cgccactcca ccggcggcat ggacgagctg tacaagttag tagcttaata atactagagc
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttatata ctagagacat tgattatttg cacggcgtca cactttgcta tgccatagca
     1021 agatagtcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
     1081 tctccatacc gtttttttgg gctagctact agagtcacac aggaaaccta ctagatgagc
     1141 acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt
     1201 tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg
     1261 atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac
     1321 gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc
     1381 agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat
     1441 gagtaccctg ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt
     1501 accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc
     1561 tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct
     1621 gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata
     1681 gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg
     1741 tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt
     1801 gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa
     1861 aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgc
//