LOCUS       BBa_K654038             1140 bp    DNA     linear       01-Jun-2024
DEFINITION  Synechocystis sigA promoter controlled Renilla Luciferase
ACCESSION   BBa_K654038
VERSION     BBa_K654038.1  
FEATURES             Location/Qualifiers
     promoter        4..45
                     /label=sigA promoter
     RBS             46..55
                     /label=putative rbs
     misc_feature    46..46
                     /label=+1 Transcription Start Point
     misc_feature    9..14
                     /label=-35 box
     misc_feature    30..35
                     /label=-10 box
     promoter        1..61
                     /label=BBa_K390018
     misc_feature    68..1003
                     /label=Rluc
     CDS             68..1003
                     /label=Rluc
     misc_feature    68..1003
                     /label=BBa_J52008
     stem_loop       1023..1066
                     /label=stem_loop
     misc_feature    1012..1091
                     /label=BBa_B0010
     terminator      1012..1091
                     /label=BBa_B0010
     stem_loop       1107..1126
                     /label=T7 TE
     misc_feature    1133..1133
                     /label=stop
     polyA_site      1127..1140
                     /label=polya
     misc_feature    1100..1140
                     /label=BBa_B0012
     terminator      1100..1140
                     /label=BBa_B0012
     terminator      1012..1140
                     /label=BBa_B0015
     misc_feature    68..1140
                     /label=BBa_K654034
ORIGIN
        1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg
       61 ctactagatg gcttccaagg tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc
      121 tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac tccttcatca actactatga
      181 ttccgagaag cacgccgaga acgccgtgat ttttctgcat ggtaacgctg cctccagcta
      241 cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct agatgcatca tccctgatct
      301 gatcggaatg ggtaagtccg gcaagagcgg gaatggctca tatcgcctcc tggatcacta
      361 caagtacctc accgcttggt tcgagctgct gaaccttcca aagaaaatca tctttgtggg
      421 ccacgactgg ggggcttgtc tggcctttca ctactcctac gagcaccaag acaagatcaa
      481 ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga
      541 catcgaggag gatatcgccc tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa
      601 taacttcttc gtcgagacca tgctcccaag caagatcatg cggaaactgg agcctgagga
      661 gttcgctgcc tacctggagc cattcaagga gaagggcgag gttagacggc ctaccctctc
      721 ctggcctcgc gagatccctc tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg
      781 caactacaac gcctaccttc gggccagcga cgatctgcct aagatgttca tcgagtccga
      841 ccctgggttc ttttccaacg ctattgtcga gggagctaag aagttcccta acaccgagtt
      901 cgtgaaggtg aagggcctcc acttcagcca ggaggacgct ccagatgaaa tgggtaagta
      961 catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag taatactaga gccaggcatc
     1021 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1081 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//