LOCUS       BBa_K654041             1857 bp    DNA     linear       01-Jun-2024
DEFINITION  Synechocystis sigA promoter controlled Firefly Luciferase
ACCESSION   BBa_K654041
VERSION     BBa_K654041.1  
FEATURES             Location/Qualifiers
     promoter        4..45
                     /label=sigA promoter
     RBS             46..55
                     /label=putative rbs
     misc_feature    46..46
                     /label=+1 Transcription Start Point
     misc_feature    9..14
                     /label=-35 box
     misc_feature    30..35
                     /label=-10 box
     promoter        1..61
                     /label=BBa_K390018
     CDS             68..1720
                     /label=BBa_I712019
     stem_loop       1740..1783
                     /label=stem_loop
     misc_feature    1729..1808
                     /label=BBa_B0010
     terminator      1729..1808
                     /label=BBa_B0010
     stem_loop       1824..1843
                     /label=T7 TE
     misc_feature    1850..1850
                     /label=stop
     polyA_site      1844..1857
                     /label=polya
     misc_feature    1817..1857
                     /label=BBa_B0012
     terminator      1817..1857
                     /label=BBa_B0012
     terminator      1729..1857
                     /label=BBa_B0015
     misc_feature    68..1857
                     /label=BBa_K654033
ORIGIN
        1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg
       61 ctactagatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga
      121 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg
      181 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga
      241 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat
      301 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat
      361 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat
      421 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa
      481 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta
      541 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga
      601 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc
      661 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag
      721 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt
      781 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg
      841 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca
      901 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag
      961 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc
     1021 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca
     1081 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa
     1141 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac
     1201 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat
     1261 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct
     1321 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct
     1381 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt
     1441 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg
     1501 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat
     1561 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt
     1621 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat
     1681 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa tactagagcc aggcatcaaa
     1741 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1801 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//