LOCUS BBa_K654087 2779 bp DNA linear 19-May-2024 DEFINITION sigA controlled Thioesterase + Acyl-CoA Synthetase ACCESSION BBa_K654087 VERSION BBa_K654087.1 FEATURES Location/Qualifiers promoter 4..45 /label=sigA promoter RBS 46..55 /label=putative rbs misc_feature 46..46 /label=+1 Transcription Start Point misc_feature 9..14 /label=-35 box misc_feature 30..35 /label=-10 box promoter 1..61 /label=BBa_K390018 misc_feature 68..70 /label=Start misc_feature 716..718 /label=Stop misc_feature 692..715 /label=8-His Tag CDS 68..691 /label=Thioesterase (TesA) CDS 68..718 /label=BBa_K654058 stem_loop 738..781 /label=stem_loop misc_feature 727..806 /label=BBa_B0010 terminator 727..806 /label=BBa_B0010 stem_loop 822..841 /label=T7 TE misc_feature 848..848 /label=stop polyA_site 842..855 /label=polya misc_feature 815..855 /label=BBa_B0012 terminator 815..855 /label=BBa_B0012 terminator 727..855 /label=BBa_B0015 misc_feature 68..855 /label=BBa_K654064 misc_feature 1..855 /label=BBa_K654076 promoter 867..908 /label=sigA promoter RBS 909..918 /label=putative rbs misc_feature 909..909 /label=+1 Transcription Start Point misc_feature 872..877 /label=-35 box misc_feature 893..898 /label=-10 box promoter 864..924 /label=BBa_K390018 misc_feature 933..935 /label=Start misc_feature 2640..2642 /label=Stop misc_feature 2616..2639 /label=8-His Tag CDS 933..2615 /label=fadD acyl-CoA synthetase CDS 933..2642 /label=BBa_K654059 stem_loop 2662..2705 /label=stem_loop misc_feature 2651..2730 /label=BBa_B0010 terminator 2651..2730 /label=BBa_B0010 stem_loop 2746..2765 /label=T7 TE misc_feature 2772..2772 /label=stop polyA_site 2766..2779 /label=polya misc_feature 2739..2779 /label=BBa_B0012 terminator 2739..2779 /label=BBa_B0012 terminator 2651..2779 /label=BBa_B0015 misc_feature 933..2779 /label=BBa_K654065 misc_feature 864..2779 /label=BBa_K654077 ORIGIN 1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg 61 ctactagatg atgaacttca acaatgtttt ccgctggcat ttgcccttcc tgttcctggt 121 cctgttaacc ttccgtgccg ccgcagcgga cacgttattg attctgggtg atagcctgag 181 cgccgggtat cgaatgtctg ccagcgcggc ctggcctgcc ttgttgaatg ataagtggca 241 gagtaaaacg tcggtagtta atgccagcat cagcggcgac acctcgcaac aaggactggc 301 gcgccttccg gctctgctga aacagcatca gccgcgttgg gtgctggttg aactgggcgg 361 caatgacggt ttgcgtggtt ttcagccaca gcaaaccgag caaacgctgc gccagatttt 421 gcaggatgtc aaagccgcca acgctgaacc attgttaatg caaatacgtc tgcctgcaaa 481 ctatggtcgc cgttataatg aagcctttag cgccatttac cccaaactcg ccaaagagtt 541 tgatgttccg ctgctgccct tttttatgga agaggtctac ctcaagccac aatggatgca 601 ggatgacggt attcatccca accgcgacgc ccagccgttt attgccgact ggatggcgaa 661 gcagttgcag cctttagtaa atcatgactc acatcatcac catcatcacc atcactaata 721 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 781 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 841 ctttctgcgt ttatatacta gagcctcaac tttgactgac cagctaattt tgtacacgac 901 ttaggagtta acgaggaaaa cggctactag agttgaagaa ggtttggctt aaccgttatc 961 ccgcggacgt tccgacggag atcaaccctg accgttatca atctctggta gatatgtttg 1021 agcagtcggt cgcgcgctac gccgatcaac ctgcgtttgt gaatatgggg gaggtaatga 1081 ccttccgcaa gctggaagaa cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg 1141 ggctgaagaa aggcgatcgc gttgcgttga tgatgcctaa tttattgcaa tatccggtgg 1201 cgctgtttgg cattttgcgt gccgggatga tcgtcgtaaa cgttaacccg ttgtataccc 1261 cgcgtgagct tgagcatcag cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta 1321 actttgctca cacactggaa aaagtggttg ataaaaccgc cgttcagcac gtaattctga 1381 cccgtatggg cgatcagcta tctacggcaa aaggcacggt agtcaatttc gttgttaaat 1441 acatcaagcg tttggtgccg aaataccatc tgccagatgc catttcattt cgtagcgcac 1501 tgcataacgg ctaccggatg cagtacgtca aacccgaact ggtgccggaa gatttagctt 1561 ttctgcaata caccggcggc accactggtg tggcgaaagg cgcgatgctg actcaccgca 1621 atatgctggc gaacctggaa caggttaacg cgacctatgg tccgctgttg catccgggca 1681 aagagctggt ggtgacggcg ctgccgctgt atcacatttt tgccctgacc attaactgcc 1741 tgctgtttat cgaactgggt gggcagaacc tgcttatcac taacccgcgc gatattccag 1801 ggttggtaaa agagttagcg aaatatccgt ttaccgctat cacgggcgtt aacaccttgt 1861 tcaatgcgtt gctgaacaat aaagagttcc agcagctgga tttctccagt ctgcatcttt 1921 ccgcaggcgg tgggatgcca gtgcagcaag tggtggcaga gcgttgggtg aaactgaccg 1981 gacagtatct gctggaaggc tatggcctta ccgagtgtgc gccgctggtc agcgttaacc 2041 catatgatat tgattatcat agtggtagca tcggtttgcc ggtgccgtcg acggaagcca 2101 aactggtgga tgatgatgat aatgaagtac caccaggtca accgggtgag ctttgtgtca 2161 aaggaccgca ggtgatgctg ggttactggc agcgtcccga tgctaccgat gaaatcatca 2221 aaaatggctg gttacacacc ggcgacatcg cggtaatgga tgaagaagga ttcctgcgca 2281 ttgtcgatcg taaaaaagac atgattctgg tttccggttt taacgtctat cccaacgaga 2341 ttgaagatgt cgtcatgcag catcctggcg tacaggaagt cgcggctgtt ggcgtacctt 2401 ccggctccag tggtgaagcg gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg 2461 aagagtcact ggtgactttt tgccgccgtc agctcacggg atacaaagta ccgaagctgg 2521 tggagtttcg tgatgagtta ccgaaatcta acgtcggaaa aattttgcga cgagaattac 2581 gtgacgaagc gcgcggcaaa gtggacaata aagcccatca tcaccatcat caccatcact 2641 gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2701 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2761 gggcctttct gcgtttata //