LOCUS       BBa_K656006             1145 bp    DNA     linear       18-May-2024
DEFINITION  IPTG Reporter Using Cohesin-Gly-Ser-Gly-Cohesin
ACCESSION   BBa_K656006
VERSION     BBa_K656006.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..75
                     /label=BBa_J61100
     misc_feature    84..518
                     /label=BBa_K656002
     misc_feature    527..538
                     /label=Short Linker
     misc_feature    527..538
                     /label=BBa_K243004
     misc_feature    547..1008
                     /label=BBa_K656004
     stem_loop       1028..1071
                     /label=stem_loop
     misc_feature    1017..1096
                     /label=BBa_B0010
     terminator      1017..1096
                     /label=BBa_B0010
     stem_loop       1112..1131
                     /label=T7 TE
     misc_feature    1138..1138
                     /label=stop
     polyA_site      1132..1145
                     /label=polya
     misc_feature    1105..1145
                     /label=BBa_B0012
     terminator      1105..1145
                     /label=BBa_B0012
     terminator      1017..1145
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg ggacatacta gaggttccgt cagacggtgt ggtagtagaa attggcaaag
      121 ttacgggatc tgttggaact acagttgaaa tacctgtata tttcagagga gttccatcca
      181 aaggaatagc aaactgcgac tttgtgttca gatatgatcc gaatgtattg gaaattatag
      241 ggatagatcc cggagacata atagttgacc cgaatcctac caagagcttt gatactgcaa
      301 tatatcctga cagaaagata atagtattct tgtttgcgga agacagcgga acaggagcgt
      361 atgcaataac taaagacgga gtatttgcaa aaataagagc aactgtaaaa tcaagtgctc
      421 cgggctatat tactttcgac gaagtaggtg gatttgcaga taatgacctg gtagaacaga
      481 aggtatcatt tatagacggt ggtgttaacg ttggcaatta ctagagggtg gttctggtta
      541 ctagaggatt ctcttaaagt tacagtagga acagctaatg gtaagcctgg cgatacagta
      601 acagttcctg ttacatttgc tgatgtagca aagatgaaaa acgtaggaac atgtaatttc
      661 tatcttggat atgatgcaag cctgttagag gtagtatcag tagatgcagg tccaatagtt
      721 aagaatgcag cagttaactt ctcaagcagt gcaagcaacg gaacaatcag cttcctgttc
      781 ttggataaca caattacaga cgaattgata acagcagacg gtgtgtttgc aaatattaag
      841 ttcaaattaa agagtgtaac ggctaaaact acaacaccag taacatttaa agatggtgga
      901 gcttttggtg acggaactat gtcaaagata gcttcagtta ctaagacaaa cggtagtgta
      961 actatagatc ctggtactca acctacaaag gaacttaaag tagcagtata ctagagccag
     1021 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1081 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1141 ttata
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