LOCUS       BBa_K663001             2299 bp    DNA     linear       10-Jun-2024
DEFINITION  Wintergreen-generator on an homoserine lactone responsive promoter
ACCESSION   BBa_K663001
VERSION     BBa_K663001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1070..1082
                     /label=RBS-3\Weak
     RBS             1076..1079
                     /label=RBS
     misc_feature    1070..1082
                     /label=BBa_B0032
     RBS             1070..1082
                     /label=BBa_B0032
     CDS             1089..2162
                     /label=BMST1
     misc_feature    1089..1091
                     /label=start codon
     misc_feature    2160..2162
                     /label=stop codon
     CDS             1089..2162
                     /label=BBa_J45004
     stem_loop       2182..2225
                     /label=stem_loop
     misc_feature    2171..2250
                     /label=BBa_B0010
     terminator      2171..2250
                     /label=BBa_B0010
     stem_loop       2266..2285
                     /label=T7 TE
     misc_feature    2292..2292
                     /label=stop
     polyA_site      2286..2299
                     /label=polya
     misc_feature    2259..2299
                     /label=BBa_B0012
     terminator      2259..2299
                     /label=BBa_B0012
     misc_feature    1070..2299
                     /label=BBa_J45119
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa
     1081 agtactagat ggaagttgtt gaagttcttc acatgaatgg aggaaatgga gacagtagct
     1141 atgcaaacaa ttctttggtt cagcaaaagg tgattctcat gacaaagcca ataactgagc
     1201 aagccatgat tgatctctac agcagcctct ttccagaaac cttatgcatt gcagatttgg
     1261 gttgttcttt gggagctaac actttcttgg tggtctcaca gcttgttaaa atagtagaaa
     1321 aagaacgaaa aaagcatggt tttaagtctc cagagtttta ttttcacttc aatgatcttc
     1381 ctggcaatga ttttaataca ctttttcagt cactgggggc atttcaagaa gatttgagaa
     1441 agcatatagg ggaaagcttt ggtccatgtt ttttcagtgg agtgcctggt tcattttata
     1501 ctagactttt cccttccaaa agtttacatt ttgtttactc ctcctacagt ctcatgtggc
     1561 tatctcaggt gcctaatggg attgaaaata acaagggaaa catttacatg gcaagaacaa
     1621 gccctctaag tgttattaaa gcatactaca agcaatatga aatagatttt tcaaattttc
     1681 tcaagtaccg ttcagaggaa ttgatgaaag gtggaaagat ggtgttaaca ctcctaggta
     1741 gagaaagtga ggatcctact agcaaagaat gctgttacat ttgggagctt ctagccatgg
     1801 ccctcaataa gttggttgaa gagggattga taaaagaaga gaaagtagat gcattcaata
     1861 ttcctcaata cacaccatca ccagcagaag taaagtacat agttgagaag gaaggatcat
     1921 tcaccattaa tcgcttggaa acatcaagag ttcattggaa tgcttctaat aatgagaaga
     1981 atggtggtta caatgtgtca aggtgcatga gagctgtggc tgagcctttg cttgtcagcc
     2041 actttgacaa ggaattgatg gatttagtgt tccacaagta cgaagagatt gtttctgatt
     2101 gcatgtccaa agagaatact gagtttataa atgtcatcat ctccttgacc aaaataaatt
     2161 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2221 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2281 gggcctttct gcgtttata
//