LOCUS       BBa_K676004             1028 bp    DNA     linear       19-May-2024
DEFINITION  Supercoilometer
ACCESSION   BBa_K676004
VERSION     BBa_K676004.1  
FEATURES             Location/Qualifiers
     misc_feature    61..61
                     /label=Transcription Start Site
     misc_feature    47..52
                     /label=-10 region
     misc_feature    26..31
                     /label=-35 region
     promoter        1..103
                     /label=tyrT promoter
     misc_feature    1..103
                     /label=BBa_K676003
     promoter        1..103
                     /label=BBa_K676003
     misc_feature    116..119
                     /label=conserved
     RBS             112..123
                     /label=BBa_B0034
     CDS             130..891
                     /label=CFP (LVA)
     misc_feature    848..885
                     /label=SsrA
     misc_feature    886..891
                     /label=2
     misc_feature    198..198
                     /label=A
     misc_feature    130..891
                     /label=BBa_E0022
     misc_feature    130..891
                     /label=BBa_E0022
     stem_loop       911..954
                     /label=stem_loop
     misc_feature    900..979
                     /label=BBa_B0010
     terminator      900..979
                     /label=BBa_B0010
     stem_loop       995..1014
                     /label=T7 TE
     misc_feature    1021..1021
                     /label=stop
     polyA_site      1015..1028
                     /label=polya
     misc_feature    988..1028
                     /label=BBa_B0012
     terminator      988..1028
                     /label=BBa_B0012
     misc_feature    112..1028
                     /label=BBa_E0422
ORIGIN
        1 cagttcattt ttctcaacgt aacactttac agcggcgcgt catttgatat gatgcgcccc
       61 gcttcccgat aagggagcag gccagtaaaa agcattaccc cgttactaga gaaagaggag
      121 aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      181 gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      241 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      301 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac
      361 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      421 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      481 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      541 ctggggcaca agctggagta caactacatc agccacaacg tctatatcac cgccgacaag
      601 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
      661 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      721 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      781 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      841 tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc
      901 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      961 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1021 cgtttata
//