LOCUS       BBa_K726009              802 bp    DNA     linear       18-May-2024
DEFINITION  T7 driven lac operated inducer for the rhl quorum-sensing system
ACCESSION   BBa_K726009
VERSION     BBa_K726009.1  
FEATURES             Location/Qualifiers
     promoter        1..17
                     /label=T7 promoter
     misc_feature    100..117
                     /label=His tag
     misc_feature    22..42
                     /label=lac operator 
     promoter        1..129
                     /label=BBa_K726003
     CDS             136..738
                     /label=rhlL
     misc_feature    739..771
                     /label=LVA
     misc_feature    778..802
                     /label=Help:Barcodes
     CDS             136..777
                     /label=BBa_C0070
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taataaggag atataccatg ggcagcagcc atcaccatca tcaccacagc
      121 caggatccgt actagatgat cgaactgctg tccgaatccc tggaaggtct gtccgctgct
      181 atgatcgctg aactgggtcg ttaccgtcac caggttttca tcgaaaaact gggttgggac
      241 gttgtttcca cctcccgtgt tcgtgaccag gagttcgacc agttcgacca cccgcagacc
      301 cgttacatcg ttgctatgtc ccgtcagggt atctgcggtt gcgctcgtct gctgccgacc
      361 accgacgctt acctgctgaa agacgttttc gcttacctgt gctccgaaac cccgccgtcc
      421 gacccgtccg tttgggaact gtcccgttac gctgcttccg ctgctgacga cccgcagctg
      481 gctatgaaaa tcttctggtc ctccctccag tgcgcttggt acctgggtgc ttcctccgtt
      541 gttgctgtta ccaccaccgc tatggaacgt tacttcgttc gtaacggtgt tatcctccag
      601 cgtctgggtc cgccgcagaa agttaaaggt gaaaccctgg ttgctatctc cttcccggct
      661 taccaggaac gtggtctgga aatgctgctg cgttaccacc cggaatggct ccagggtgtt
      721 ccgctgtcca tggctgttgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc
      781 tgatagtgct agtgtagatc tc
//
LOCUS       BBa_K726008              802 bp    DNA     linear       18-May-2024
DEFINITION  T7 driven lac operated inducer for the rhl quorum-sensing system
ACCESSION   BBa_K726008
VERSION     BBa_K726008.1  
FEATURES             Location/Qualifiers
     promoter        1..17
                     /label=T7 promoter
     misc_feature    100..117
                     /label=His tag
     misc_feature    22..42
                     /label=lac operator 
     promoter        1..129
                     /label=BBa_K726003
     CDS             136..738
                     /label=rhlL
     misc_feature    739..771
                     /label=LVA
     misc_feature    778..802
                     /label=Help:Barcodes
     CDS             136..802
                     /label=BBa_C0070
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
       61 tgtttaactt taataaggag atataccatg ggcagcagcc atcaccatca tcaccacagc
      121 caggatccgt actagatgat cgaactgctg tccgaatccc tggaaggtct gtccgctgct
      181 atgatcgctg aactgggtcg ttaccgtcac caggttttca tcgaaaaact gggttgggac
      241 gttgtttcca cctcccgtgt tcgtgaccag gagttcgacc agttcgacca cccgcagacc
      301 cgttacatcg ttgctatgtc ccgtcagggt atctgcggtt gcgctcgtct gctgccgacc
      361 accgacgctt acctgctgaa agacgttttc gcttacctgt gctccgaaac cccgccgtcc
      421 gacccgtccg tttgggaact gtcccgttac gctgcttccg ctgctgacga cccgcagctg
      481 gctatgaaaa tcttctggtc ctccctccag tgcgcttggt acctgggtgc ttcctccgtt
      541 gttgctgtta ccaccaccgc tatggaacgt tacttcgttc gtaacggtgt tatcctccag
      601 cgtctgggtc cgccgcagaa agttaaaggt gaaaccctgg ttgctatctc cttcccggct
      661 taccaggaac gtggtctgga aatgctgctg cgttaccacc cggaatggct ccagggtgtt
      721 ccgctgtcca tggctgttgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc
      781 tgatagtgct agtgtagatc tc
//