LOCUS       BBa_K727500             2575 bp    DNA     linear       23-May-2024
DEFINITION  TMT Coding Device
ACCESSION   BBa_K727500
VERSION     BBa_K727500.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..65
                     /label=prefix
     misc_feature    237..237
                     /label=T
     misc_feature    555..555
                     /label=A
     CDS             64..813
                     /label=luxR
     misc_feature    64..819
                     /label=BBa_C0062
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             64..819
                     /label=BBa_C0062
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     misc_feature    46..981
                     /label=BBa_I0462
     misc_feature    990..1009
                     /label=LuxR/HSL
     promoter        1009..1014
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1042..1042
                     /label=start
     misc_feature    990..1044
                     /label=BBa_R0062
     promoter        990..1044
                     /label=BBa_R0062
     misc_feature    1..1044
                     /label=BBa_J09855
     CDS             1053..2575
                     /label=TMT
     misc_feature    1053..2575
                     /label=BBa_K727003
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag aggaattcgc ggccgcttct agatggcaaa
     1081 aaataatgca gttgcaggtt ttaatgcact gaatggtgtt gaactgaatc tgtttaccac
     1141 cgatgaactg aaagcaattc attatgcaac catggaagtt ctgatggatc cgggtattca
     1201 ggttagcgat ccggaagcac gtcagatttt taaagaaaat ggttgtgaag ttaatgaaaa
     1261 aaccaatgtt gttaaaattc cggaatatct ggttcgtaaa gcattacagc tggcaccgag
     1321 ccgttttgtt ctgtggggtc gtgataaaaa atttaatacc gttcaggaat gtggtggtaa
     1381 agttcattgg acctgttttg gtaccggtgt taaagtttgt aaatatcagg atggtaaata
     1441 tgttaccgtt gatagcgttg aaaaagatat tgcagatatt gcaaaactgt gtgattgggc
     1501 agaaaatatt gattatttta gcctgccggt tagcgcacgt gatattgcag gtcagggtgc
     1561 acaggatgtt catgaaaccc tgaccccgct ggcaaatacc gcaaaacatt ttcatcatat
     1621 tgatccggtt ggtgaaaatg ttgaatatta tcgtgatatt gttaaagcat attatggtgg
     1681 tgatgaagaa gaagcacgta aaaaaccgat ttttagcatg ctgctgtgtc cgaccagccc
     1741 gctggaactg agcgttaatg catgtcaggt tattattaaa ggtgcacgtt ttggtattcc
     1801 ggttaatgtt ctgagcatgg caatgagcgg tggtagcagc ccggtttatc tggcaggtac
     1861 cctggttacc cataatgcag aagttctgag cggtattgtt ctggcacagc tgaccgttcc
     1921 gggtgcaaaa gtttggtatg gtagcagcac caccaccttt gatctgaaaa aaggtaccgc
     1981 accggttggt agcccggaac tgggtctgat tagcgcagca gttgcaaaac tggcacagtt
     2041 ttatggtctg ccgagctatg ttgcaggtag cagcgatgca aaagttccgg atgatcaggc
     2101 aggtcatgaa aaaaccatga ccaccctgct gccggcactg gcaggtgcaa ataccattta
     2161 tggtgcaggt atgctggaac tgggtatgac ctttagcatg gaacagctgg ttattgataa
     2221 tgatattttt agcatggtta aaaaagcaat gcagggtatt ccggttagcg aagaaaccct
     2281 ggcagttgaa agcattcaga aagttggtat tggtaataat tttctggcac tgaaacagac
     2341 ccgtcagctg gttgattatc cgagcaatcc gatgctgctg gatcgtcata tgtttggtga
     2401 ttgggcagca gcaggtagca aagatctggc aaccgttgca catgaaaaag ttgaagatgt
     2461 tctgaaaaat catcaggtta ccccgattga tgcagatatt tttaaagata tgcaggcaat
     2521 tgttgataaa gcagataaag catttcgtgg tatgtactag tagcggccgc tgcag
//