BBa_C0061
1
luxI
autoinducer synthetase for AHL
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
Synthesizes 3OC<sub>6</sub>HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2213985
1
Help:Barcodes
range2213985
1
619
643
annotation7038
1
BBa_C0061
range7038
1
1
618
annotation1761
1
luxI
range1761
1
1
579
annotation1760
1
LVA
range1760
1
580
611
BBa_K727622
1
BBa_K727622
Rose Odor Generator Device (Geraniol)-Complete System
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genome of
GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production.GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production. We added Lux cassette right promoter that up-regulated by HSL and LuxR in the course of being able to regulate easily. We intended to generate a positive feedback response with the help of producing LuxR and HSL and activating the promoter with them.
false
false
_973_
0
11153
9
Not in stock
false
Optimized for E.coli
false
İBRAHİM YASİR ORHAN
component2259350
1
BBa_K727006
component2259342
1
BBa_R0062
component2259348
1
BBa_K727005
component2259358
1
BBa_C0061
component2259353
1
BBa_C0062
annotation2259353
1
BBa_C0062
range2259353
1
2869
3624
annotation2259348
1
BBa_K727005
range2259348
1
64
1004
annotation2259358
1
BBa_C0061
range2259358
1
3656
4273
annotation2259350
1
BBa_K727006
range2259350
1
1013
2862
annotation2259342
1
BBa_R0062
range2259342
1
1
55
BBa_K727006
1
BBa_K727006
Geraniol Synthase
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genom on
GS (Geraniol syntase) synthesizes Geraniol which is the second reaction of geraniol production.
false
false
_973_
0
11153
9
Not in stock
true
Optimized for E.coli
false
İBRAHİM YASİR ORHAN
annotation2176260
1
GS
range2176260
1
1
1850
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2046
1
-35
range2046
1
20
25
annotation2045
1
LuxR/HSL
range2045
1
1
20
annotation2047
1
-10
range2047
1
42
47
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2048
1
start
range2048
1
53
53
BBa_K727005
1
BBa_K727005
Geranyl diphosphate Synthase
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genom on
GPPS (Geranyl diphosphate synthase) synthesizes GPP (Geranyl diphosphate) which is the first reaction of geraniol production.
false
false
_973_
0
11153
9
Not in stock
true
Optemized for E.coli
false
İBRAHİM YASİR ORHAN
annotation2176259
1
GPPS
range2176259
1
1
941
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
true
_1_
0
24
7
In stock
false
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
annotation1766
1
luxR
range1766
1
1
750
annotation1764
1
T
range1764
1
174
174
annotation1762
1
prefix
range1762
1
1
2
annotation1765
1
A
range1765
1
492
492
BBa_C0061_sequence
1
atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_K727005_sequence
1
gaattcgcggccgcttctagatggattttccgcagcagctggaagcatgtgttaaacaggcaaatcaggcactgagccgttttattgcaccgctgccgtttcagaataccccggttgttgaaaccatgcagtatggcgcactgctgggtggtaaacgtctgcgtccgtttctggtttatgcaaccggtcatatgtttggtgttagcaccaataccctggatgcaccggcagcagcagttgaatgtattcatgcatatagcctgattcatgatgatctgccggcaatggatgatgatgatctgcgtcgtggtctgccgacctgtcatgttaaatttggcgaagcaaatgcaattctggcaggtgatgcacttcagaccctggcatttagcattctgagcgatgcagatatgccggaagttagcgatcgtgatcgtattagcatgattagcgaactggcaagcgcaagcggtattgcaggtatgtgtggtggtcaggcactggatctggatgcagaaggtaaacatgttccgctggatgcactggaacgtattcatcgtcataaaaccggtgcactgattcgtgcagcagttcgtctgggtgcactgagcgcaggtgataaaggtcgtcgtgcactgccggttctggataaatatgcagaaagcattggtctggcatttcaggttcaggatgatattctggatgttgttggtgataccgcaaccctgggtaaacgtcagggtgcagatcagcagctgggtaaaagcacctatccggcactgctgggtctggaacaggcacgtaaaaaagcacaggatctgattgatgatgcacgtcagagcctgaaacagctggcagaacagagcattgataccagcgcactggaagcactggcagattatattattcagcgtaataaataatactagtagcggccgctgcag
BBa_K727006_sequence
1
gaattcgcggccgcttctagatgagcagcattagccagaaagttgttattggtctgaataaagcagcagcaaataataatttacagaatctggatcgtcgtggttttaaaacccgttgtgttagcagcagcaaagcagcaagctgtctgcgtgcaagctgtagcttacagctggatgttaaaccggttcaggaaggtcgtcgtagcggtaattatcagccgagcatttgggattttaattatgttcagagcctgaataccccgtataaagaagaacgttatctgacccgtcatgcagaactgattgttcaggttaaaccgctgctggaaaaaaaaatggaaccggcacagcagctggaactgattgatgatctgaataatctgggtctgagctatttttttcaggatcgtattaaacagattctgagctttatttatgatgaaaatcagtgttttcatagcaatattaatgatcaggcagaaaaacgtgatctgtattttaccgcactgggttttcgtctgctgcgtcagcatggttttgatgttagccaggaagtttttgattgttttaaaaatgataatggtagcgattttaaagcaagcctgagcgataataccaaaggtctgttacagctgtatgaagcaagctttctggttcgtgaaggtgaagataccctggaacaggcacgtcagtttgcaaccaagtttctgcgtcgtaaactggatgaaattgatgataatcatctgctgagctgtattcatcatagcctggagattccgctgcattggcgtattcagcgtctggaagcacgttggtttctggatgcatatgcaacccgtcatgatatgaatccggttattctggaactggcaaaactggattttaatattattcaggcaacccatcaggaagaactgaaagatgttagccgttggtggcagaatacccgtctggcagaaaaactgccgtttgttcgtgatcgtctggttgaaagctatttttgggcaattgcactgtttgaaccgcatcagtatggttatcagcgtcgtgttgcagcaaaaattattaccctggcaaccagcattgatgatgtttatgatatttatggtaccctggatgaattacagctgtttaccgataattttcgtcgttgggataccgaaagcctgggtcgtctgccgtatagcatgcagctgttttatatggttattcataattttgttagcgaactggcatatgagattctgaaagaaaaaggttttattgttattccgtatttacagcgtagctgggttgatctggcagaaagctttctgaaagaagcaaattggtattatagcggttataccccgagcctggaagaatatattgataatggtagcattagcattggtgcagttgcagttctgagccaggtttattttaccctggcaaatagcattgaaaaaccgaaaattgaaagcatgtataaatatcatcatattctgcgtctgagcggtctgctggttcgtctgcatgatgatctgggtaccagcctgtttgaaaaaaaacgtggtgatgttccgaaagcagttgagatttgtatgaaagaacgtaatgttaccgaagaagaagcagaagaacatgttaaatatctgattcgtgaagcatggaaagaaatgaataccgcaaccaccgcagcaggttgtccgtttatggatgaactgaatgttgcagcagcaaatctgggtcgtgcagcacagtttgtttatctggatggtgatggtcatggtgttcagcatagcaagattcatcagcagatgggtggtctgatgtttgaaccgtatgtttactagtagcggccgctgcag
BBa_K727622_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggaattcgcggccgcttctagatggattttccgcagcagctggaagcatgtgttaaacaggcaaatcaggcactgagccgttttattgcaccgctgccgtttcagaataccccggttgttgaaaccatgcagtatggcgcactgctgggtggtaaacgtctgcgtccgtttctggtttatgcaaccggtcatatgtttggtgttagcaccaataccctggatgcaccggcagcagcagttgaatgtattcatgcatatagcctgattcatgatgatctgccggcaatggatgatgatgatctgcgtcgtggtctgccgacctgtcatgttaaatttggcgaagcaaatgcaattctggcaggtgatgcacttcagaccctggcatttagcattctgagcgatgcagatatgccggaagttagcgatcgtgatcgtattagcatgattagcgaactggcaagcgcaagcggtattgcaggtatgtgtggtggtcaggcactggatctggatgcagaaggtaaacatgttccgctggatgcactggaacgtattcatcgtcataaaaccggtgcactgattcgtgcagcagttcgtctgggtgcactgagcgcaggtgataaaggtcgtcgtgcactgccggttctggataaatatgcagaaagcattggtctggcatttcaggttcaggatgatattctggatgttgttggtgataccgcaaccctgggtaaacgtcagggtgcagatcagcagctgggtaaaagcacctatccggcactgctgggtctggaacaggcacgtaaaaaagcacaggatctgattgatgatgcacgtcagagcctgaaacagctggcagaacagagcattgataccagcgcactggaagcactggcagattatattattcagcgtaataaataatactagtagcggccgctgcagtactagaggaattcgcggccgcttctagatgagcagcattagccagaaagttgttattggtctgaataaagcagcagcaaataataatttacagaatctggatcgtcgtggttttaaaacccgttgtgttagcagcagcaaagcagcaagctgtctgcgtgcaagctgtagcttacagctggatgttaaaccggttcaggaaggtcgtcgtagcggtaattatcagccgagcatttgggattttaattatgttcagagcctgaataccccgtataaagaagaacgttatctgacccgtcatgcagaactgattgttcaggttaaaccgctgctggaaaaaaaaatggaaccggcacagcagctggaactgattgatgatctgaataatctgggtctgagctatttttttcaggatcgtattaaacagattctgagctttatttatgatgaaaatcagtgttttcatagcaatattaatgatcaggcagaaaaacgtgatctgtattttaccgcactgggttttcgtctgctgcgtcagcatggttttgatgttagccaggaagtttttgattgttttaaaaatgataatggtagcgattttaaagcaagcctgagcgataataccaaaggtctgttacagctgtatgaagcaagctttctggttcgtgaaggtgaagataccctggaacaggcacgtcagtttgcaaccaagtttctgcgtcgtaaactggatgaaattgatgataatcatctgctgagctgtattcatcatagcctggagattccgctgcattggcgtattcagcgtctggaagcacgttggtttctggatgcatatgcaacccgtcatgatatgaatccggttattctggaactggcaaaactggattttaatattattcaggcaacccatcaggaagaactgaaagatgttagccgttggtggcagaatacccgtctggcagaaaaactgccgtttgttcgtgatcgtctggttgaaagctatttttgggcaattgcactgtttgaaccgcatcagtatggttatcagcgtcgtgttgcagcaaaaattattaccctggcaaccagcattgatgatgtttatgatatttatggtaccctggatgaattacagctgtttaccgataattttcgtcgttgggataccgaaagcctgggtcgtctgccgtatagcatgcagctgttttatatggttattcataattttgttagcgaactggcatatgagattctgaaagaaaaaggttttattgttattccgtatttacagcgtagctgggttgatctggcagaaagctttctgaaagaagcaaattggtattatagcggttataccccgagcctggaagaatatattgataatggtagcattagcattggtgcagttgcagttctgagccaggtttattttaccctggcaaatagcattgaaaaaccgaaaattgaaagcatgtataaatatcatcatattctgcgtctgagcggtctgctggttcgtctgcatgatgatctgggtaccagcctgtttgaaaaaaaacgtggtgatgttccgaaagcagttgagatttgtatgaaagaacgtaatgttaccgaagaagaagcagaagaacatgttaaatatctgattcgtgaagcatggaaagaaatgaataccgcaaccaccgcagcaggttgtccgtttatggatgaactgaatgttgcagcagcaaatctgggtcgtgcagcacagtttgtttatctggatggtgatggtcatggtgttcagcatagcaagattcatcagcagatgggtggtctgatgtttgaaccgtatgtttactagtagcggccgctgcagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_C0062_sequence
1
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z