LOCUS       BBa_K727632             1770 bp    DNA     linear       27-May-2024
DEFINITION  Constitutive LuxR Promoter With GFP
ACCESSION   BBa_K727632
VERSION     BBa_K727632.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..65
                     /label=prefix
     misc_feature    237..237
                     /label=T
     misc_feature    555..555
                     /label=A
     CDS             64..813
                     /label=luxR
     misc_feature    64..819
                     /label=BBa_C0062
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             64..819
                     /label=BBa_C0062
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     misc_feature    46..981
                     /label=BBa_I0462
     misc_feature    990..1009
                     /label=LuxR/HSL
     promoter        1009..1014
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1042..1042
                     /label=start
     misc_feature    990..1044
                     /label=BBa_R0062
     promoter        990..1044
                     /label=BBa_R0062
     misc_feature    1..1044
                     /label=BBa_J09855
     CDS             1051..1770
                     /label=GFP protein
     CDS             1051..1770
                     /label=BBa_E0040
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag atgcgtaaag gagaagaact tttcactgga
     1081 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     1141 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
     1201 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
     1261 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     1321 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     1381 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     1441 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     1501 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     1561 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     1621 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     1681 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     1741 catggcatgg atgaactata caaataataa
//