BBa_K727777
1
BBa_K727777
FMO3 Generator-Complete system
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genom of
FMO3 (flavin monooxygenase 3) is an enzyme that catalyses the oxidation of TMA (trimethylamine) into TMAO (trimethylamine oxide) which results as the removal of unpleasant fish odor.
false
false
_973_
0
11153
9
Not in stock
false
Optimized for E.coli
false
İBRAHİM YASİR ORHAN
component2251009
1
BBa_K727004
component2251017
1
BBa_C0061
component2251012
1
BBa_C0062
component2251003
1
BBa_R0062
annotation2251009
1
BBa_K727004
range2251009
1
64
1700
annotation2251012
1
BBa_C0062
range2251012
1
1707
2462
annotation2251003
1
BBa_R0062
range2251003
1
1
55
annotation2251017
1
BBa_C0061
range2251017
1
2494
3111
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
true
_1_
0
24
7
In stock
false
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation1766
1
luxR
range1766
1
1
750
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation1765
1
A
range1765
1
492
492
annotation1764
1
T
range1764
1
174
174
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
annotation1762
1
prefix
range1762
1
1
2
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2046
1
-35
range2046
1
20
25
annotation2048
1
start
range2048
1
53
53
annotation2047
1
-10
range2047
1
42
47
annotation2045
1
LuxR/HSL
range2045
1
1
20
BBa_K727004
1
BBa_K727004
Flavin monooxygenase 3
2012-06-15T11:00:00Z
2015-05-08T01:13:05Z
Genom on
FMO3 (flavin monooxygenase 3) is an enzyme that catalyses the oxidation of TMA (trimethylamine) into TMAO (trimethylamine oxide) which results as the removal of unpleasant fish odor.
false
false
_973_
0
11153
9
Not in stock
false
Optimized for E.coli
false
İBRAHİM YASİR ORHAN
annotation2176258
1
FMO3
range2176258
1
1
1637
BBa_C0061
1
luxI
autoinducer synthetase for AHL
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
Synthesizes 3OC<sub>6</sub>HSL, which binds to LuxR.</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. . <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation1761
1
luxI
range1761
1
1
579
annotation1760
1
LVA
range1760
1
580
611
annotation7038
1
BBa_C0061
range7038
1
1
618
annotation2213985
1
Help:Barcodes
range2213985
1
619
643
BBa_C0061_sequence
1
atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_K727004_sequence
1
gaattcgcggccgcttctagatgggtaaaaaagttgcaattattggtgcaggtgttagcggtctggcaagcattcgtagctgtctggaagaaggtctggaaccgacctgttttgaaaaaagcaatgatattggtggtctgtggaaatttagcgatcatgcagaagaaggtcgtgcaagcatttataaaagcgtttttagcaatagcagcaaagaaatgatgtgttttccggattttccgtttccggatgattttccgaattttatgcataatagcaaaattcaggaatatattattgcatttgcaaaagaaaaaaatctgctgaaatatattcagtttaaaacctttgttagcagcgttaataaacatccggattttgcaaccaccggtcagtgggatgttaccaccgaacgtgatggtaaaaaagaaagcgcagtttttgatgcagttatggtttgtagcggtcatcatgtttatccgaatctgccgaaagaaagctttccgggtctgaatcattttaaaggtaaatgttttcatagccgtgattataaagaaccgggtgtttttaatggtaaacgtgttctggttgttggtctgggtaatagcggttgtgatattgcaaccgaactgagccgtaccgcagaacaggttatgattagcagccgtagcggtagctgggttatgagccgtgtttgggataatggttatccgtgggatatgctgctggttacccgttttggtacctttctgaaaaataatctgccgaccgcaattagcgattggctgtatgttaaacagatgaatgcacgttttaaacatgaaaattatggtctgatgccgctgaatggtgttctgcgtaaagaaccggtttttaatgatgaactgccggcaagcattctgtgtggtattgttagcgttaaaccgaatgttaaagaatttaccgaaaccagcgcaatttttgaagatggtaccatttttgaaggtattgattgtgttatttttgcaaccggttatagctttgcatatccgtttctggatgaaagcattattaaaagccgtaataatgaaattattctgtttaaaggtgtttttccgccgctgctggaaaaaagcaccattgcagttattggttttgttcagagcctgggtgcagcaattccgaccgttgatctgcagagccgttgggcagcacaggttattaaaggtacctgtaccctgccgagcatggaagatatgatgaatgatattaatgaaaaaatggaaaaaaaacgtaaatggtttggtaaaagcgaaaccattcagaccgattatattgtttatatggatgaactgagcagctttattggtgcaaaaccgaatattccgtggctgtttctgaccgatccgaaactggcaatggaagtttattttggtccgtgtagcccgtatcagtttcgtctggttggtccgggtcagtggccgggtgcacgtaatgcaattctgacccagtgggatcgtagcctgaaaccgatgcagacccgtgttgttggtcgtctgcagaaaccgtgttttttttttcattggctgaaactgtttgcaattccgattctgctgattgcagtttttctggttctgacctactagtagcggccgctgcag
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_K727777_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagaggaattcgcggccgcttctagatgggtaaaaaagttgcaattattggtgcaggtgttagcggtctggcaagcattcgtagctgtctggaagaaggtctggaaccgacctgttttgaaaaaagcaatgatattggtggtctgtggaaatttagcgatcatgcagaagaaggtcgtgcaagcatttataaaagcgtttttagcaatagcagcaaagaaatgatgtgttttccggattttccgtttccggatgattttccgaattttatgcataatagcaaaattcaggaatatattattgcatttgcaaaagaaaaaaatctgctgaaatatattcagtttaaaacctttgttagcagcgttaataaacatccggattttgcaaccaccggtcagtgggatgttaccaccgaacgtgatggtaaaaaagaaagcgcagtttttgatgcagttatggtttgtagcggtcatcatgtttatccgaatctgccgaaagaaagctttccgggtctgaatcattttaaaggtaaatgttttcatagccgtgattataaagaaccgggtgtttttaatggtaaacgtgttctggttgttggtctgggtaatagcggttgtgatattgcaaccgaactgagccgtaccgcagaacaggttatgattagcagccgtagcggtagctgggttatgagccgtgtttgggataatggttatccgtgggatatgctgctggttacccgttttggtacctttctgaaaaataatctgccgaccgcaattagcgattggctgtatgttaaacagatgaatgcacgttttaaacatgaaaattatggtctgatgccgctgaatggtgttctgcgtaaagaaccggtttttaatgatgaactgccggcaagcattctgtgtggtattgttagcgttaaaccgaatgttaaagaatttaccgaaaccagcgcaatttttgaagatggtaccatttttgaaggtattgattgtgttatttttgcaaccggttatagctttgcatatccgtttctggatgaaagcattattaaaagccgtaataatgaaattattctgtttaaaggtgtttttccgccgctgctggaaaaaagcaccattgcagttattggttttgttcagagcctgggtgcagcaattccgaccgttgatctgcagagccgttgggcagcacaggttattaaaggtacctgtaccctgccgagcatggaagatatgatgaatgatattaatgaaaaaatggaaaaaaaacgtaaatggtttggtaaaagcgaaaccattcagaccgattatattgtttatatggatgaactgagcagctttattggtgcaaaaccgaatattccgtggctgtttctgaccgatccgaaactggcaatggaagtttattttggtccgtgtagcccgtatcagtttcgtctggttggtccgggtcagtggccgggtgcacgtaatgcaattctgacccagtgggatcgtagcctgaaaccgatgcagacccgtgttgttggtcgtctgcagaaaccgtgttttttttttcattggctgaaactgtttgcaattccgattctgctgattgcagtttttctggttctgacctactagtagcggccgctgcagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
BBa_C0062_sequence
1
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z