LOCUS       BBa_K731255             2089 bp    DNA     linear       17-May-2024
DEFINITION  Inducible araC-pBAD promoter with GFPmut3b, weak RBS
ACCESSION   BBa_K731255
VERSION     BBa_K731255.1  
FEATURES             Location/Qualifiers
     promoter        1080..1203
                     /label=pBAD
     promoter        1043..1114
                     /label=PC
     CDS             1..1080
                     /label=araC
     promoter        1..1203
                     /label=BBa_K731201
     RBS             1212..1226
                     /label=RBS-1\Strong
     RBS             1219..1223
                     /label=RBS
     misc_feature    1212..1226
                     /label=BBa_B0030
     RBS             1212..1226
                     /label=BBa_B0030
     CDS             1233..1952
                     /label=GFP protein
     CDS             1233..1952
                     /label=BBa_E0040
     stem_loop       1972..2015
                     /label=stem_loop
     misc_feature    1961..2040
                     /label=BBa_B0010
     terminator      1961..2040
                     /label=BBa_B0010
     stem_loop       2056..2075
                     /label=T7 TE
     misc_feature    2082..2082
                     /label=stop
     polyA_site      2076..2089
                     /label=polya
     misc_feature    2049..2089
                     /label=BBa_B0012
     terminator      2049..2089
                     /label=BBa_B0012
     misc_feature    1212..2089
                     /label=BBa_E0840
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg
      181 actaatgcgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgtactaga gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg
     1261 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     1321 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1381 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
     1441 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
     1501 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     1561 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     1621 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     1681 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
     1741 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     1801 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     1861 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     1921 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
     1981 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2041 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//