Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Francesco Guzzonato
Date created: 2012-08-21 11:00:00
Date modified: 2015-05-08 01:13:06
[LacI + LacIq promoter] reverse + [tac promoter + lac operator] forward
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K731500_sequence (Version 1) |
Description
This is E.coli lactose-inducibile promoter.lac repressor (encoded from lacI gene) is the regulator protein: in absence of lactose the protein binds to lac operator, preventing RNA-polymerase binding and transcription.
Low, constitutive levels of transcription can be seen anyhow.
Source
pET21b| Sequence Annotation | Location | Component / Role(s) |
| stop LacI start rbs LacIq promoter BBa_K731300 lacO operator Ptac promoter | 1,3 1,1084 1081,1084 1085,1092 1093,1153 1,1153 1194,1219 1162,1188 | feature/stop stop_codon CDS feature/cds start_codon feature/start feature/rbs ribosome_entry_site promoter feature/promoter feature/BioBrick engineered_region operator feature/operator feature/promoter promoter |