BBa_K731722

BBa_K731722 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K731722
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Depetris, Giacomo Giacomelli
Date created: 2012-08-22 11:00:00
Date modified: 2015-05-08 01:13:06

T1 terminator from E. coli rrnB



Types
DnaRegion

Roles
terminator

Terminator

Sequences BBa_K731722_sequence (Version 1)

Description

This is the B0010 part extracted by PCR from the doulbe terminator B0012.
We didn't manage to extract it directly from the registry and we discovered that others had the same problem; for this reason we are submitting it once again, confirmed by sequencing and by experience...ready to use!
The characterization has been done both with T7 and Coli RNApolymerases.

Notes

I don't have any design considerations, anyway it is useful to use TBE1X instead of TAE1X to make screening gels of such small fragments.

Source

This part comes from B0012 contained in E0840, a double terminator in which B0010 is the second one. We used an extraction PCR.

Sequence Annotation Location Component / Role(s)
B0010
1,79
feature/misc sequence_feature
igem#experience
Works
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K731722/1