LOCUS       BBa_K736002              928 bp    DNA     linear       26-May-2024
DEFINITION  Constitutive Promoter with MLC-binding-site-regulated enhanced RFP
ACCESSION   BBa_K736002
VERSION     BBa_K736002.1  
FEATURES             Location/Qualifiers
     promoter        1..59
                     /label=BBa_K736000
     misc_feature    72..75
                     /label=conserved
     RBS             68..79
                     /label=BBa_B0034
     CDS             86..760
                     /label=mrfp1
     misc_feature    767..791
                     /label=Help:Barcodes
     CDS             86..766
                     /label=BBa_E1010
     stem_loop       811..854
                     /label=stem_loop
     misc_feature    800..879
                     /label=BBa_B0010
     terminator      800..879
                     /label=BBa_B0010
     stem_loop       895..914
                     /label=T7 TE
     misc_feature    921..921
                     /label=stop
     polyA_site      915..928
                     /label=polya
     misc_feature    888..928
                     /label=BBa_B0012
     terminator      888..928
                     /label=BBa_B0012
     misc_feature    68..928
                     /label=BBa_I13507
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagcattaa ttacgaagcg caaaaaaatt
       61 actagagaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
      121 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
      181 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
      241 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
      301 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
      361 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
      421 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
      481 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
      541 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
      601 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
      661 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
      721 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
      781 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      841 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      901 ccttcgggtg ggcctttctg cgtttata
//