LOCUS       BBa_K737004             2545 bp    DNA     linear       23-May-2024
DEFINITION  Inducible GVPA Generator
ACCESSION   BBa_K737004
VERSION     BBa_K737004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     misc_feature    44..1351
                     /label=BBa_P0412
     misc_feature    1362..1378
                     /label=lac O1
     promoter        1379..1384
                     /label=-35
     misc_feature    1385..1401
                     /label=lac O1
     promoter        1402..1407
                     /label=-10
     misc_feature    1360..1413
                     /label=BBa_R0011
     promoter        1360..1413
                     /label=BBa_R0011
     misc_feature    1427..1430
                     /label=conserved
     RBS             1423..1434
                     /label=BBa_B0034
     CDS             1441..1659
                     /label=BBa_K737016
     RBS             1668..1682
                     /label=RBS-1\Strong
     RBS             1675..1679
                     /label=RBS
     misc_feature    1668..1682
                     /label=BBa_B0030
     RBS             1668..1682
                     /label=BBa_B0030
     CDS             1689..2408
                     /label=GFP protein
     CDS             1689..2408
                     /label=BBa_E0040
     stem_loop       2428..2471
                     /label=stem_loop
     misc_feature    2417..2496
                     /label=BBa_B0010
     terminator      2417..2496
                     /label=BBa_B0010
     stem_loop       2512..2531
                     /label=T7 TE
     misc_feature    2538..2538
                     /label=stop
     polyA_site      2532..2545
                     /label=polya
     misc_feature    2505..2545
                     /label=BBa_B0012
     terminator      2505..2545
                     /label=BBa_B0012
     misc_feature    1668..2545
                     /label=BBa_E0840
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt
     1381 gacattgtga gcggataaca agatactgag cacatactag agaaagagga gaaatactag
     1441 atggccgttg aaaaagtaaa ctcatcctcc agtctggccg aagttatcga tcggatctta
     1501 gataaaggca ttgtgattga cgcttgggta cgggtttccc tcgttggaat cgagcttcta
     1561 tccatagaag caagaatcgt gatcgcttct gttgaaacct atctcaagta cgcagaagcc
     1621 gttggtttaa ccgcacaggc ggctgttcct tcggtctaat actagagatt aaagaggaga
     1681 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1741 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1801 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1861 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1921 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1981 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     2041 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     2101 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     2161 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     2221 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     2281 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     2341 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     2401 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2461 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2521 tcgggtgggc ctttctgcgt ttata
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