RS2

BBa_K743001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K743001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Date created: 2012-09-19 11:00:00

Date modified: 2015-05-08 01:13:09

Synechocystis PCC6803 neutral recombination site.

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K743001_sequence (Version 1)

Description

When placed between this part and BBa_K743000, any DNA sequence will be inserted into Synechocystis PCC6803 chromosome trough homologous recombination

Notes

According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.

Source

PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RS2	1,687	sequence_feature, feature/dna

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K743001/1

Member of these Collections

• iGEM Parts Registry