LOCUS       BBa_K743004             3307 bp    DNA     linear       14-Jun-2024
DEFINITION  psb1C3_IntK plasmid with RFP under psaAB promoter
ACCESSION   BBa_K743004
VERSION     BBa_K743004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=RS1 recombination site
     misc_feature    1..522
                     /label=BBa_K743000
     promoter        531..569
                     /label=psaAB operon promoter
     misc_feature    570..572
                     /label=+1 Transcription Start Point(s)
     misc_feature    699..707
                     /label=rbs region (non exact)
     misc_feature    535..540
                     /label=-35 box
     misc_feature    557..562
                     /label=-10 box
     promoter        523..715
                     /label=BBa_K390016
     CDS             716..1390
                     /label=mrfp1
     misc_feature    1397..1421
                     /label=Help:Barcodes
     CDS             716..1421
                     /label=BBa_E1010
     stem_loop       1433..1476
                     /label=stem_loop
     misc_feature    1422..1501
                     /label=BBa_B0010
     terminator      1422..1501
                     /label=BBa_B0010
     stem_loop       1517..1536
                     /label=T7 TE
     misc_feature    1543..1543
                     /label=stop
     polyA_site      1537..1550
                     /label=polya
     misc_feature    1510..1550
                     /label=BBa_B0012
     terminator      1510..1550
                     /label=BBa_B0012
     terminator      1422..1550
                     /label=BBa_B0015
     CDS             1699..2517
                     /label=kanamycin resistance
     misc_feature    1551..2517
                     /label=BBa_P1003
     stem_loop       2533..2552
                     /label=T7 TE
     misc_feature    2559..2559
                     /label=stop
     polyA_site      2553..2566
                     /label=polya
     misc_feature    2526..2566
                     /label=BBa_B0012
     terminator      2526..2566
                     /label=BBa_B0012
     stem_loop       2587..2609
                     /label=stem_loop
     misc_feature    2575..2620
                     /label=BBa_B0011
     terminator      2575..2620
                     /label=BBa_B0011
     misc_feature    1551..2620
                     /label=BBa_K292003
     misc_feature    2621..3307
                     /label=RS2
     misc_feature    2621..3307
                     /label=BBa_K743001
ORIGIN
        1 tcccagcctc tcaaccaccg atattgggcc cctagctacc cctgatattt tggcggacat
       61 tgttgcgcaa gtagaacaaa ccattgctgc tggagcccac tgtcgttgtg gcggccaagc
      121 cctagaccaa ccgggaaatt actatcctcc caccctgctc accgacgttc cccccaacgc
      181 ccctacctac cgtcaggaat tttttggccc cgtggccctc ggatttactg tcgataattt
      241 agaggaggcg atcgccttgg ccaatgacat tccctttggg ttgggggcca gtgcatggac
      301 aactaacccg gaaaatcaac aaaaactaat ccggggcata gaagccgggg ctgtattcat
      361 taacggtatg actaaatctg acccccgcat tccctttgga ggcatcaagc gctctggctt
      421 tgggcgggaa cttggccgca tgggcatttt agaatttgtc aatgctaaaa ccgtttggat
      481 tgcttagtcc gccaccattt taaaattatc gctcttctat tcgttacgca ggtcttgcct
      541 aggggggggg aggccgtatt atcttctagt gatgtttgct gaaaacgcct atctgtgcaa
      601 ggtttaacat cgttattatg aagcgaaaac taattccctt ttttacgctt cctctattac
      661 actattctgc ataggaaacc cttaatagtt cattgtcgag cgaggagaac cctgcatggc
      721 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      781 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      841 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      901 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      961 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     1021 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1081 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1141 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     1201 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1261 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1321 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1381 caccggtgct taataacgct gatagtgcta gtgtagatcg cccaggcatc aaataaaacg
     1441 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1501 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata ctgatccttc
     1561 aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa tctctgatgt
     1621 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     1681 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc ccgtccgcgc
     1741 ttaaactcca acatggacgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1801 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1861 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtccg tctcaactgg
     1921 ctgacggagt ttatgcctct cccgaccatc aagcatttta tccgtactcc tgatgatgcg
     1981 tggttactca ccaccgcgat tcctgggaaa acagccttcc aggtattaga agaatatcct
     2041 gattcaggtg aaaatattgt tgatgcgctg gccgtgttcc tgcgccggtt acattcgatt
     2101 cctgtttgta attgtccttt taacagcgat cgtgtatttc gtcttgctca ggcgcaatca
     2161 cgcatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2221 gttgaacaag tctggaaaga aatgcacaag ctcttgccat tctcaccgga ttcagtcgtc
     2281 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2341 attgatgttg gacgggtcgg aatcgcagac cgttaccagg accttgccat tctttggaac
     2401 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2461 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaataatac
     2521 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
     2581 tataaaaagc cagattatta atccggcttt tttattattt agtgtaggcg gtaaagtcta
     2641 tgggaacttc cagtttaagg gctgtcccgt taaatatggc ttcgagtttt tgaagcataa
     2701 ttttctaacg tctgtggatt tttttaagca tagcttgacc ttctgttggt cgaattctag
     2761 ggattttcat ccatattcgg ttcatagggc agctcaatgg tgaacattgc tcccccagtt
     2821 tcatggttag ctgcggttaa atttcccctg tgggccagga caatttcctt ggcgatcgcc
     2881 aaacccaggc cactgccatg gcgttgacta tcgggatgta ggcgagcacg ggaactatca
     2941 ccccgataaa gtcgctcaaa aatgtagggt aaatcccttg gttgaaatcc taggccttga
     3001 tccctaataa tcatggtgat cccttctgtg ccttgatgac cctgcacaaa aatagtccct
     3061 tccggtgggc tatatttcag cgtattgtcc aaaatattca tcaatacctg catcagacga
     3121 tcgccatccc cctctaggtt caacttggtt ggcccttggt aatccagggt gatatttttt
     3181 accacggcga tcggggttaa tcgctcccag gtaatggcga gtaaatggcg taaattaatc
     3241 ggttctggct cgaggtaaag attgggatta gcggttattt gggttaaatg gagccaactt
     3301 tccacca
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