LOCUS       BBa_K743008             2313 bp    DNA     linear       02-Jun-2024
DEFINITION  psb1C3_IntKR recombination plasmid for Synechocystis PCC6803
ACCESSION   BBa_K743008
VERSION     BBa_K743008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=RS1 recombination site
     misc_feature    1..522
                     /label=BBa_K743000
     stem_loop       534..577
                     /label=stem_loop
     misc_feature    523..602
                     /label=BBa_B0010
     terminator      523..602
                     /label=BBa_B0010
     stem_loop       618..637
                     /label=T7 TE
     misc_feature    644..644
                     /label=stop
     polyA_site      638..651
                     /label=polya
     misc_feature    611..651
                     /label=BBa_B0012
     terminator      611..651
                     /label=BBa_B0012
     terminator      523..651
                     /label=BBa_B0015
     CDS             660..1478
                     /label=kanamycin resistance
     misc_feature    1446..1446
                     /label=G to C mutation to eliminate XhoI site
     misc_feature    1583..1626
                     /label=repeat region
     misc_feature    660..1626
                     /label=BBa_P1007
     misc_feature    523..1626
                     /label=BBa_S05073
     misc_feature    1627..2313
                     /label=RS2
     misc_feature    1627..2313
                     /label=BBa_K743001
ORIGIN
        1 tcccagcctc tcaaccaccg atattgggcc cctagctacc cctgatattt tggcggacat
       61 tgttgcgcaa gtagaacaaa ccattgctgc tggagcccac tgtcgttgtg gcggccaagc
      121 cctagaccaa ccgggaaatt actatcctcc caccctgctc accgacgttc cccccaacgc
      181 ccctacctac cgtcaggaat tttttggccc cgtggccctc ggatttactg tcgataattt
      241 agaggaggcg atcgccttgg ccaatgacat tccctttggg ttgggggcca gtgcatggac
      301 aactaacccg gaaaatcaac aaaaactaat ccggggcata gaagccgggg ctgtattcat
      361 taacggtatg actaaatctg acccccgcat tccctttgga ggcatcaagc gctctggctt
      421 tgggcgggaa cttggccgca tgggcatttt agaatttgtc aatgctaaaa ccgtttggat
      481 tgcttagtcc gccaccattt taaaattatc gctcttctat tcccaggcat caaataaaac
      541 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      601 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt
      661 tattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
      721 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
      781 caaagaatgg caaggtcctg gtaacggtct gcgattccga cccgtccaac atcaatacaa
      841 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
      901 actgaatccg gtgagaatgg caagagcttg tgcatttctt tccagacttg ttcaacaggc
      961 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt catgcgtgat
     1021 tgcgcctgag caagacgaaa tacacgatcg ctgttaaaag gacaattaca aacaggaatc
     1081 gaatgtaacc ggcgcaggaa cacggccagc gcatcaacaa tattttcacc tgaatcagga
     1141 tattcttcta atacctggaa ggctgttttc ccaggaatcg cggtggtgag taaccacgca
     1201 tcatcaggag tacggataaa atgcttgatg gtcgggagag gcataaactc cgtcagccag
     1261 ttgagacgga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     1321 aacaactctg gcgcatcggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg
     1381 acattatcgc gagcccattt atacccatat aaatcagcgt ccatgttgga gtttaagcgc
     1441 ggacgggagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     1501 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     1561 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
     1621 gatcagagtg taggcggtaa agtctatggg aacttccagt ttaagggctg tcccgttaaa
     1681 tatggcttcg agtttttgaa gcataatttt ctaacgtctg tggatttttt taagcatagc
     1741 ttgaccttct gttggtcgaa ttctagggat tttcatccat attcggttca tagggcagct
     1801 caatggtgaa cattgctccc ccagtttcat ggttagctgc ggttaaattt cccctgtggg
     1861 ccaggacaat ttccttggcg atcgccaaac ccaggccact gccatggcgt tgactatcgg
     1921 gatgtaggcg agcacgggaa ctatcacccc gataaagtcg ctcaaaaatg tagggtaaat
     1981 cccttggttg aaatcctagg ccttgatccc taataatcat ggtgatccct tctgtgcctt
     2041 gatgaccctg cacaaaaata gtcccttccg gtgggctata tttcagcgta ttgtccaaaa
     2101 tattcatcaa tacctgcatc agacgatcgc catccccctc taggttcaac ttggttggcc
     2161 cttggtaatc cagggtgata ttttttacca cggcgatcgg ggttaatcgc tcccaggtaa
     2221 tggcgagtaa atggcgtaaa ttaatcggtt ctggctcgag gtaaagattg ggattagcgg
     2281 ttatttgggt taaatggagc caactttcca cca
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