BBa_K743001 1 RS2 Synechocystis PCC6803 neutral recombination site. 2012-09-19T11:00:00Z 2015-05-08T01:13:09Z PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337 When placed between this part and BBa_K743000, any DNA sequence will be inserted into Synechocystis PCC6803 chromosome trough homologous recombination false false _994_ 0 11584 9 It's complicated false According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size. false annotation2188524 1 RS2 range2188524 1 1 687 BBa_K743000 1 RS1 Synechocystis PCC6803 neutral recombination site. 2012-09-19T11:00:00Z 2015-05-08T01:13:09Z PCR amplified from synechocystis PCC6803 chromosome. Any DNA sequence flacked by this part and BBa_K743001 will be incorporated into Synechocistys PCC6803 chromosome through a neutral double homologous recombination. false false _994_ 0 11584 9 In stock false According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size. false annotation2188518 1 RS1 recombination site range2188518 1 1 522 BBa_I746916 1 BBa_I746916 superfolder GFP coding sequence 2008-09-29T11:00:00Z 2015-08-31T04:08:05Z Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart). This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven). false false _116_ 0 2122 9 It's complicated false Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis. false Stefan Milde annotation1977533 1 start range1977533 1 1 3 annotation1977534 1 superfolder GFP coding region range1977534 1 1 720 annotation1977535 1 stop range1977535 1 715 720 BBa_M0050 1 LAA AANDENYALAA. (Very fast) SsrA degradation tag. 2007-12-05T12:00:00Z 2015-05-08T01:13:51Z C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli. This sequence codes for the amino acid sequence AANDENYALAA, which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~5 Vmax/ [Clpx6] min-1 from (1) ~0.5%/min on log scale from (2) ~1 min half life from (3) See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240. false false _11_ 0 2398 11 Not in stock false C-terminal tag. Degradation rate is very fast. Deviations from this sequence in key amino acids will lower degradation rates (see Parts BBa_M0051, BBa_M0052, BBa_M0053). Three C-terminal aa's (LAA in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX. false Felix Moser annotation1958880 1 WT SsrA tag AANDENYALAA range1958880 1 1 33 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K743003 1 BBa_K743003 Pta promoter and native rbs from Synechocystis PCC6803 2012-09-19T11:00:00Z 2015-05-08T01:13:09Z This part was PCR amplified from Synechocystis chromosome, orf slr1739. This is the 150bp sequence upstream the Transaldolase gene start codon, it contains putative rbs and promoter regions. Transaldolase is an enzyme involved in the pentose phospate cycle and its mRNA levels have shown to oscillate in a circadian manner, peaking at hour 14. false false _994_ 0 11584 9 Not in stock false It was assumed that the 150bp sequence upstream from the start codon includes the promoter and the ribosome binding site false Juan Sim??n ??lamos annotation2188682 1 Pta range2188682 1 1 150 BBa_K743018 1 sfGFP.degr sfGFP.degradation.tag under Pta promoter in psb1C3_IntKR plasmid 2012-09-22T11:00:00Z 2015-05-08T01:13:09Z false false _994_ 0 11584 9 It's complicated false false component2194652 1 BBa_K743000 component2194660 1 BBa_M0050 component2194658 1 BBa_I746916 component2194674 1 BBa_K743001 component2194654 1 BBa_K743003 component2194672 1 BBa_S05073 annotation2194652 1 BBa_K743000 range2194652 1 1 522 annotation2194658 1 BBa_I746916 range2194658 1 687 1406 annotation2194672 1 BBa_S05073 range2194672 1 1456 2559 annotation2194654 1 BBa_K743003 range2194654 1 531 680 annotation2194660 1 BBa_M0050 range2194660 1 1415 1447 annotation2194674 1 BBa_K743001 range2194674 1 2568 3254 BBa_P1007 1 KanR kanamycin resistance cassette 2006-02-03T12:00:00Z 2015-05-08T01:14:11Z This contains KanR, a terminator and then AmpR and a terminator. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation1919401 1 repeat region range1919401 1 924 967 annotation1919400 1 G to C mutation to eliminate XhoI site range1919400 1 787 787 annotation1919399 1 kanamycin resistance range1919399 1 1 819 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_S05073 1 BBa_S05073 B0015:P1007 2012-09-19T11:00:00Z 2015-05-08T01:14:49Z false false _9_ 0 11584 9 Not in stock false false Juan Sim??n ??lamos component2188873 1 BBa_P1007 component2188869 1 BBa_B0015 annotation2188873 1 BBa_P1007 range2188873 1 138 1104 annotation2188869 1 BBa_B0015 range2188869 1 1 129 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K743001_sequence 1 agtgtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcacaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccatccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggttaaatggagccaactttccacca BBa_K743018_sequence 1 tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattctactagagaacgcatcggcgttccctagcttaaattatcttgatgtccaaagaaggctcgctttcgggaatcaccattaccattggagggaaagacgttgaacttgtttaatcgccattatgggtaagaatctcctcgaacaactgcgccaatttacatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagaggctgctaacgacgaaaactacgctctggctgcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagtactagagagtgtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcacaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccatccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggttaaatggagccaactttccacca BBa_K743000_sequence 1 tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattc BBa_I746916_sequence 1 atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga BBa_K743003_sequence 1 aacgcatcggcgttccctagcttaaattatcttgatgtccaaagaaggctcgctttcgggaatcaccattaccattggagggaaagacgttgaacttgtttaatcgccattatgggtaagaatctcctcgaacaactgcgccaatttaca BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M0050_sequence 1 gctgctaacgacgaaaactacgctctggctgct BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_P1007_sequence 1 ttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcag BBa_S05073_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z