LOCUS       BBa_K749024             1398 bp    DNA     linear       01-Jun-2024
DEFINITION  consecutively produces PFDH, formaldehyde dehydrogenase from 
            Pseudomonas putida
ACCESSION   BBa_K749024
VERSION     BBa_K749024.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1261
                     /label=BBa_K749005
     stem_loop       1281..1324
                     /label=stem_loop
     misc_feature    1270..1349
                     /label=BBa_B0010
     terminator      1270..1349
                     /label=BBa_B0010
     stem_loop       1365..1384
                     /label=T7 TE
     misc_feature    1391..1391
                     /label=stop
     polyA_site      1385..1398
                     /label=polya
     misc_feature    1358..1398
                     /label=BBa_B0012
     terminator      1358..1398
                     /label=BBa_B0012
     terminator      1270..1398
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtctggc aatcgtggag tggtatatct cggcgccggc aaggtcgaag tgcaacacat
      121 cgactacccg aaaatgcagg acccgcgcgg caagaaaatc gaacacggcg tcatcctgaa
      181 ggtggtctcc accaacatct gcggttctga ccagcacatg gtccgtggcc gcaccactgc
      241 ccaggtcggc ctggtcctgg gccacgaaat caccggtgaa atcgtcgaga agggccgcga
      301 cgtcgagcgc atgcagatcg gcgacctggt atcggtaccg ttcaacgtcg cctgcggccg
      361 ctgccgttcc tgcaaagaga tgcatactgg tgtctgcctc accgtcaacc cggcccgcgc
      421 cggcggtgcc tacggctacg tcgacatggg cgactggacc ggtggccagg ccgaatacgt
      481 gctggtgcca tacgccgact tcaacctgct gaagctgcct gagcgcgaca aggccatgga
      541 aaagatccgt gacctgacct gcctgtccga catcctgccc accggctacc acggtgccgt
      601 aacggcaggc gtaggcccag gcagcactgt ctacgttgcc ggtgccggcc cggtcggcct
      661 ggccgccgct gcctcggcgc gcctgctggg cgccgcctgc gtaatcgtcg gtgacctcaa
      721 cccggcccgc ctggcccacg ccaagtcgca aggcttcgaa gtggtcgacc tgtccaagga
      781 caccccgctg cacgagcaga tcgtcgacat cctcggcgag ccggaagtgg actgcgctgt
      841 cgacgccgta ggcttcgagg cccgtggcca cggccatgaa ggtgccaagc acgaggcccc
      901 ggccaccgtg cttaactcgc tgatgcaagt gacccgcgtg gccggcaaca tcggtatccc
      961 gggcctgtac gtaaccgaag acccaggcgc ggtggatgct gcagccaaga tcggcgcgct
     1021 gagcatccgc tttggcctgg gctgggccaa gtcgcacagc ttccacaccg gccagacccc
     1081 gaccatgaaa tacaaccgcc agctgatgca ggcgatcatg tgggatcgca tcaacattgc
     1141 tgaagtggtg ggtgtgcagg tgatcaacct ggatcaggcg ccggaagggt atggcgagtt
     1201 tgatgcgggt gtgcctaaga agtttgtgat tgacccgcac aagatgtggg gtgcggcgta
     1261 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1321 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1381 ggcctttctg cgtttata
//