LOCUS BBa_K750115 3938 bp DNA linear 19-May-2024 DEFINITION pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva) ACCESSION BBa_K750115 VERSION BBa_K750115.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 160..867 /label=cI lambda misc_feature 868..900 /label=LVA misc_feature 907..931 /label=Help:Barcodes CDS 157..931 /label=BBa_C0051 stem_loop 951..994 /label=stem_loop misc_feature 940..1019 /label=BBa_B0010 terminator 940..1019 /label=BBa_B0010 stem_loop 1035..1054 /label=T7 TE misc_feature 1061..1061 /label=stop polyA_site 1055..1068 /label=polya misc_feature 1028..1068 /label=BBa_B0012 terminator 1028..1068 /label=BBa_B0012 terminator 940..1068 /label=BBa_B0015 misc_feature 1..1068 /label=BBa_K750104 promoter 1077..1111 /label=BBa_J23119 misc_feature 1124..1127 /label=conserved RBS 1120..1131 /label=BBa_B0034 CDS 1141..1757 /label=tetR misc_feature 1758..1791 /label=SsrA misc_feature 1798..1822 /label=Help:Barcodes CDS 1138..1797 /label=BBa_C0040 stem_loop 1842..1885 /label=stem_loop misc_feature 1831..1910 /label=BBa_B0010 terminator 1831..1910 /label=BBa_B0010 stem_loop 1926..1945 /label=T7 TE misc_feature 1952..1952 /label=stop polyA_site 1946..1959 /label=polya misc_feature 1919..1959 /label=BBa_B0012 terminator 1919..1959 /label=BBa_B0012 misc_feature 1968..1986 /label=TetR 1 misc_feature 1968..2021 /label=BBa_R0040 promoter 1987..1992 /label=-35 misc_feature 1993..2011 /label=TetR 2 promoter 2010..2015 /label=-10 promoter 1968..2021 /label=BBa_R0040 misc_feature 1120..2021 /label=BBa_Q04400 misc_feature 2034..2037 /label=conserved RBS 2030..2041 /label=BBa_B0034 CDS 2051..2758 /label=cI lambda misc_feature 2759..2791 /label=LVA misc_feature 2798..2822 /label=Help:Barcodes CDS 2048..2822 /label=BBa_C0051 stem_loop 2842..2885 /label=stem_loop misc_feature 2831..2910 /label=BBa_B0010 terminator 2831..2910 /label=BBa_B0010 stem_loop 2926..2945 /label=T7 TE misc_feature 2952..2952 /label=stop polyA_site 2946..2959 /label=polya misc_feature 2919..2959 /label=BBa_B0012 terminator 2919..2959 /label=BBa_B0012 terminator 2831..2959 /label=BBa_B0015 misc_feature 1077..2959 /label=BBa_K750102 promoter 3005..3010 /label=-10 promoter 2982..2987 /label=-35 misc_feature 2992..3008 /label=OR1 misc_feature 2968..2984 /label=OR2 misc_feature 2968..3016 /label=BBa_R0051 promoter 2968..3016 /label=BBa_R0051 misc_feature 3029..3032 /label=conserved RBS 3025..3036 /label=BBa_B0034 misc_feature 3043..3045 /label=start misc_feature 3796..3798 /label=stop misc_feature 3799..3801 /label=stop misc_feature 3759..3795 /label=LVA tag CDS 3046..3758 /label=cds misc_feature 3043..3801 /label=BBa_K145015 stem_loop 3821..3864 /label=stem_loop misc_feature 3810..3889 /label=BBa_B0010 terminator 3810..3889 /label=BBa_B0010 stem_loop 3905..3924 /label=T7 TE misc_feature 3931..3931 /label=stop polyA_site 3925..3938 /label=polya misc_feature 3898..3938 /label=BBa_B0012 terminator 3898..3938 /label=BBa_B0012 terminator 3810..3938 /label=BBa_B0015 misc_feature 2968..3938 /label=BBa_K750103 misc_feature 1077..3938 /label=BBa_K750114 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 181 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 241 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 301 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 361 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 421 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 481 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 541 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 601 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 661 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 721 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 781 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 841 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 901 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 961 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1021 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga 1081 cagctagctc agtcctaggt ataatgctag ctactagaga aagaggagaa atactagatg 1141 tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga 1201 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg 1261 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat 1321 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat 1381 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta 1441 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc 1501 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact 1561 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct 1621 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt 1681 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt 1741 aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac 1801 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 1861 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1921 acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc ctatcagtga 1981 tagagattga catccctatc agtgatagag atactgagca ctactagaga aagaggagaa 2041 atactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg 2101 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc 2161 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt 2221 aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag 2281 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact 2341 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga 2401 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag 2461 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa 2521 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg 2581 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga 2641 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga 2701 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc 2761 tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc 2821 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2881 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2941 gggcctttct gcgtttatat actagagtaa caccgtgcgt gttgactatt ttacctctgg 3001 cggtgataat ggttgctact agagaaagag gagaaatact agatgcgtaa aggagaagaa 3061 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 3121 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 3181 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 3241 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 3301 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 3361 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 3421 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 3481 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa 3541 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact 3601 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc 3661 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct 3721 gctgggatta cacatggcat ggatgaacta tacaaaaggc ctgctgcaaa cgacgaaaac 3781 tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 3841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 3901 cactggctca ccttcgggtg ggcctttctg cgtttata //