BBa_K753000 1 BBa_K753000 Yeast FIG1 promoter 2012-08-28T11:00:00Z 2015-05-08T01:13:12Z The sequence has been amplified directly from genomic yeast DNA. Promoter of the FIG1 gene in yeast. The transcription factor Ste12, activated in the yeast pheromone pathway, binds to the FIG1 promoter and initiate the expression of the FIG1 gene. Five pheromone response elements (PRE, consensus sequence ATGAAACA) have been identifi?ed in the 5'-upstream regulatory sequence of the FIG1 gene. Phosphorylated Ste12 binds to them and thereby activates transcription. The PREs in the FIG1 promoter di?ffer slightly from the PRE consensus sequence ATGAAACA: Sequence Location TGAAACG -405 TGAAACC* -217 TGACACA* -206 TGAAACG -173 TGAAATA -133 Sites marked with an asterisk occur on the opposite strand The strictly regulated transcriptional feature of the FIG1 promoter can be useful when investigating the pheromone pathway. The promoter can be used to express reporter genes in response to activation of the pheromone pathway. Reference: Erdman S, Lin L, Malczynski M, Snyder M. Pheromone-regulated Genes Required for Yeast Mating Diff?erentiation. The Journal of cell biology. 1998;140(3):461-483. false false _1004_ 0 11572 9 It's complicated false The length of the promoter was chosen to 450 bp as recommended by Kondo group of Kobe University of Japan. Togawa S, Ishii J, Ishikura A, Tanaka T, Ogino C, Kondo A. Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 in signalling. J Biochem. 2010 Jun;147(6):867-73 false Rachel Engeler BBa_K753000_sequence 1 atcaccctgcattgcctctttatttgacgttgtttttgtagaacatgaaacgaattttgacttgatgagacgaagtatatatccaaagaataccttaaatagaaaaggaaagataataaatactaaacactactatatattcaggtaaaatacaaaaattataacatttttaaaactttttttttttgaaagtccttctcgctttaggattttttcccattaagattatgatggtttcatgtatgtgtcagttaaaaaaaatatggctaagtagcaatgaaacgaaccaagaaaatgaaaacaatatatagtgctgttgaaataacaaagacattggtatatatttgtaaaatgtctgttaaatgttttttatctcaggttcttgcttgtctttggtagaagaaattatagtaaacaaacaaacaaacaaacaaaaaaaaaaaaaaaaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z