LOCUS BBa_K759013 4729 bp DNA linear 01-Jun-2024 DEFINITION P3HB + eYFP producing construct ACCESSION BBa_K759013 VERSION BBa_K759013.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 83..1871 /label=BBa_K342001 misc_feature 1884..1887 /label=conserved RBS 1880..1891 /label=BBa_B0034 CDS 1898..3079 /label=BBa_K759004 misc_feature 3092..3095 /label=conserved RBS 3088..3099 /label=BBa_B0034 CDS 3106..3843 /label=BBa_K759005 misc_feature 63..3843 /label=BBa_K759009 misc_feature 3856..3859 /label=conserved RBS 3852..3863 /label=BBa_B0034 CDS 3870..4592 /label=BBa_E0030 stem_loop 4612..4655 /label=stem_loop misc_feature 4601..4680 /label=BBa_B0010 terminator 4601..4680 /label=BBa_B0010 stem_loop 4696..4715 /label=T7 TE misc_feature 4722..4722 /label=stop polyA_site 4716..4729 /label=polya misc_feature 4689..4729 /label=BBa_B0012 terminator 4689..4729 /label=BBa_B0012 misc_feature 3852..4729 /label=BBa_E0430 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag agaaggaggt atataatggc gaccggcaaa ggcgcggcag 121 cttccacgca ggaaggcaag tcccaaccat tcaaggtcac gccggggcca ttcgatccag 181 ccacatggct ggaatggtcc cgccagtggc agggcactga aggcaacggc cacgcgaccg 241 cgtccggcat tccgggcctg gatgcgctgg caggcgtcaa gatcgcgccg gcgcagctgg 301 gtgatatcca gcagcgctac atgaaggact tctcagcgct gtggcaggcc atggccgagg 361 gcaaggccga ggccaccggt ccgctgcacg accggcgctt cgccggcgac gcatggcgca 421 ccaacctccc atatcgcttc gctgccgcgt tctacctgct caatgcgcgc gccttgaccg 481 agctggccga tgccgtcgag gccgatgcca agacccgcca gcgcatccgc ttcgcgatct 541 cgcaatgggt cgatgcgatg tcgcccgcca acttccttgc caccaatccc gaggcgcagc 601 gcctgctgat cgagtcgggc ggcgaatcgc tgcgtgccgg cgtgcgcaac atgatggaag 661 acctgacacg cggcaagatc tcgcagaccg acgagagcgc gtttgaggtc ggccgcaatg 721 tcgcggtgac cgaaggcgcc gtggtcttcg agaacgagta cttccagctg ttgcagtaca 781 agccgctgac cgacaaggtg cacgcgcgcc cgctgctgat ggtgccgccg tgcatcaaca 841 agtactacat cctggacatg cagccggaga gctcgctggt gcgccatgtg gtggagcagg 901 gacatacggt gtttctggtg tcgtggcgca atccggacgc cagcatggcc ggcagcacct 961 gggacgacta catcgagcac gcggccatcc gcgccatcga agtcgcgcgc gacatcagcg 1021 gccaggacaa gatcaacgtg ctcggcttct gcgtgggcgg caccattgtc tcgaccgcgc 1081 tggcggtgct ggccgcgcgc ggcgagcacc cggccgccag cgtcacgctg ctgaccacgc 1141 tgctggactt tgccgacacg ggcatcctcg acgtctttgt cgacgagggc catgtgcagt 1201 tgcgcgaggc cacgctgggc ggcggcgccg gcgcgccgtg cgcgctgctg cgcggccttg 1261 agctggccaa taccttctcg ttcttgcgcc cgaacgacct ggtgtggaac tacgtggtcg 1321 acaactacct gaagggcaac acgccggtgc cgttcgacct gctgttctgg aacggcgacg 1381 ccaccaacct gccggggccg tggtactgct ggtacctgcg ccacacctac atgcagaacg 1441 agctcaaggt accgggcaag ctgaccgtgt gcggcgtgcc ggtggacctg gccagcatcg 1501 acgtgccgac ctatatctac ggctcgcgcg aagaccatat cgtgccgtgg accgcggcct 1561 atgcctcgac cgcgctgctg gcgaacaagc tgcgcttcgt gctgggtgcg tcgggccata 1621 tcgccggtgt gatcaacccg ccggccaaga acaagcgcag ccactggact aacgatgcgc 1681 tgccggagtc gccgcagcaa tggctggccg gcgccatcga gcatcacggc agctggtggc 1741 cggactggac cgcatggctg gccgggcagg ccggcgcgaa acgcgccgcg cccgccaact 1801 atggcaatgc gcgctatcgc gcaatcgaac ccgcgcctgg gcgatacgtc aaagccaagg 1861 catgataata atactagaga aagaggagaa atactagatg actgacgttg tcatcgtatc 1921 cgccgcccgc accgcggtcg gcaagtttgg cggctcgctg gccaagatcc cggcaccgga 1981 actgggtgcc gtggtcatca aggccgcgct ggagcgcgcc ggcgtcaagc cggagcaggt 2041 gagcgaagtc atcatgggcc aggtgctgac cgccggttcg ggccagaacc ccgcacgcca 2101 ggccgcgatc aaggccggcc tgccggcgat ggtgccggcc atgaccatca acaaggtgtg 2161 cggctcgggc ctgaaggccg tgatgctggc cgccaacgcg atcatggcgg gcgacgccga 2221 gatcgtggtg gccggcggcc aggaaaacat gagcgccgcc ccgcacgtgc tgccgggctc 2281 gcgcgatggt ttccgcatgg gcgatgccaa gctggtcgac accatgatcg tcgacggcct 2341 gtgggacgtg tacaaccagt accacatggg catcaccgcc gagaacgtgg ccaaggaata 2401 cggcatcaca cgcgaggcgc aggatgagtt cgccgtcggc tcgcagaaca aggccgaagc 2461 cgcgcagaag gccggcaagt ttgacgaaga gatcgtcccg gtgctgatcc cgcagcgcaa 2521 gggcgacccg gtggccttca agaccgacga gttcgtgcgc cagggcgcca cgctggacag 2581 catgtccggc ctcaagcccg ccttcgacaa ggccggcacg gtgaccgcgg ccaacgcctc 2641 gggcctgaac gacggcgccg ccgcggtggt ggtgatgtcg gcggccaagg ccaaggaact 2701 gggcctgacc ccgctggcca cgatcaagag ctatgccaac gccggtgtcg atcccaaggt 2761 gatgggcatg ggcccggtgc cggcctccaa gcgcgccctg tcgcgcgccg agtggacccc 2821 gcaagacctg gacctgatgg agatcaacga ggcctttgcc gcgcaggcgc tggcggtgca 2881 ccagcagatg ggctgggaca cctccaaggt caatgtgaac ggcggcgcca tcgccatcgg 2941 ccacccgatc ggcgcgtcgg gctgccgtat cctggtgacg ctgctgcacg agatgaagcg 3001 ccgtgacgcg aagaagggcc tggcctcgct gtgcatcggc ggcggcatgg gcgtggcgct 3061 ggcagtcgag cgcaaatact actagagaaa gaggagaaat actagatgac tcagcgcatt 3121 gcgtatgtga ccggcggcat gggtggtatc ggaaccgcca tttgccagcg gctggccaag 3181 gatggctttc gtgtggtggc cggttgcggc cccaactcgc cgcgccgcga aaagtggctg 3241 gagcagcaga aggccctggg cttcgatttc attgcctcgg aaggcaatgt ggctgactgg 3301 gactcgacca agaccgcatt cgacaaggtc aagtccgagg tcggcgaggt tgatgtgctg 3361 atcaacaacg ccggtatcac ccgcgacgtg gtgttccgca agatgacccg cgccgactgg 3421 gatgcggtga tcgacaccaa cctgacctcg ctgttcaacg tcaccaagca ggtgatcgac 3481 ggcatggccg accgtggctg gggccgcatc gtcaacatct cgtcggtgaa cgggcagaag 3541 ggccagttcg gccagaccaa ctactccacc gccaaggccg gcctgcatgg cttcaccatg 3601 gcactggcgc aggaagtggc gaccaagggc gtgaccgtca acacggtctc tccgggctat 3661 atcgccaccg acatggtcaa ggcgatccgc caggacgtgc tcgacaagat cgtcgcgacg 3721 atcccggtca agcgcctggg cctgccggaa gagatcgcct cgatctgcgc ctggttgtcg 3781 tcggaggagt ccggtttctc gaccggcgcc gacttctcgc tcaacggcgg cctgcatatg 3841 ggctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 3901 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 3961 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 4021 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat 4081 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg 4141 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 4201 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 4261 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 4321 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 4381 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 4441 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 4501 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 4561 ctctcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga 4621 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 4681 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //