LOCUS       BBa_K764033             1731 bp    DNA     linear       28-May-2024
DEFINITION  Tetr promoter_rbs_ompA-sacB-Term
ACCESSION   BBa_K764033
VERSION     BBa_K764033.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     misc_feature    83..183
                     /label=BBa_K764027
     RBS             192..194
                     /label=RBS
     CDS             204..1625
                     /label=Coding sequence
     misc_feature    402..407
                     /label=Removed EcoRI site
     misc_feature    192..1628
                     /label=BBa_K322921
     stem_loop       1644..1663
                     /label=T7 TE
     misc_feature    1670..1670
                     /label=stop
     polyA_site      1664..1677
                     /label=polya
     misc_feature    1637..1677
                     /label=BBa_B0012
     terminator      1637..1677
                     /label=BBa_B0012
     stem_loop       1698..1720
                     /label=stem_loop
     misc_feature    1686..1731
                     /label=BBa_B0011
     terminator      1686..1731
                     /label=BBa_B0011
     terminator      1637..1731
                     /label=BBa_B0014
     misc_feature    192..1731
                     /label=BBa_K764024
     misc_feature    83..1731
                     /label=BBa_K764028
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agaattcgcg gccgcttcta gatgaagaag acagcaatag
      121 ccattgcagt cgcacttgca ggattcgcga ccgtcgccca agccactagt agcggccgct
      181 gcatactaga ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat
      241 taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg aaagaaacga
      301 accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat gatatgctgc
      361 aaatccctga acagcaaaaa aatgaaaaat atcaagttcc tgagttcgat tcgtccacaa
      421 ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca ttacaaaacg
      481 ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgcatta gccggagatc
      541 ctaaaaatgc ggatgacaca tcgatttaca tgttctatca aaaagtcggc gaaacttcta
      601 ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg
      661 attctatcct aaaagaccaa acacaagaat ggtcaggttc agccacattt acatctgacg
      721 gaaaaatccg tttattctac actgatttct ccggtaaaca ttacggcaaa caaacactga
      781 caactgcaca agttaacgta tcagcatcag acagctcttt gaacatcaac ggtgtagagg
      841 attataaatc aatctttgac ggtgacggaa aaacgtatca aaatgtacag cagttcatcg
      901 atgaaggcaa ctacagctca ggcgacaacc atacgctgag agatcctcac tacgtagaag
      961 ataaaggcca caaatactta gtatttgaag caaacactgg aactgaagat ggctaccaag
     1021 gcgaagaatc tttatttaac aaagcatact atggcaaaag cacatcattc ttccgtcaag
     1081 aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc tgagttagca aacggcgctc
     1141 tcggtatgat tgagctaaac gatgattaca cactgaaaaa agtgatgaaa ccgctgattg
     1201 catctaacac agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat
     1261 ggtacctgtt cactgactcc cgcggatcaa aaatgacgat tgacggcatt acgtctaacg
     1321 atatttacat gcttggttat gtttctaatt ctttaactgg cccatacaag ccgctgaaca
     1381 aaactggcct tgtgttaaaa atggatcttg atcctaacga tgtaaccttt acttactcac
     1441 acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat tacaagctat atgacaaaca
     1501 gaggattcta cgcagacaaa caatcaacgt ttgcgccaag cttcctgctg aacatcaaag
     1561 gcaagaaaac atctgttgtc aaagacagca tccttgaaca aggacaatta acagttaaca
     1621 aataataata ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
     1681 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt t
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