BBa_K766000
1
BBa_K766000
AHL synthetase LuxI with His-tag
2012-09-21T11:00:00Z
2015-05-08T01:13:14Z
This part is from BBa_C0261. We make a truncation on the C-terminal to cut off the LVA tag. His tag and thrombin cutting site is add on the N-terminal.
This part consists of LuxI synthase with His-tag at the N-terminal of the protein. With the help of His-tag, you can detect the expression of this enzyme. Besides, you can purify the protein using Ni-colume and gel filtraion. As a result, you can build a system to product C6HSL in vitro. Between the His-tag and protein, we add the cutting site of thrombin. So that you can cut off the his-tag from the protein.
false
false
_1018_
0
8494
9
It's complicated
false
We want to find a way to detect the expression and purify LuxI protein.
false
Yu Zhao
annotation2193941
1
Poly-hig tag
range2193941
1
13
30
annotation2193942
1
LuxI without LVA tag
range2193942
1
70
639
annotation2193943
1
thrombin cutting site
range2193943
1
40
57
BBa_K766000_sequence
1
atgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z