BBa_K766000 1 BBa_K766000 AHL synthetase LuxI with His-tag 2012-09-21T11:00:00Z 2015-05-08T01:13:14Z This part is from BBa_C0261. We make a truncation on the C-terminal to cut off the LVA tag. His tag and thrombin cutting site is add on the N-terminal. This part consists of LuxI synthase with His-tag at the N-terminal of the protein. With the help of His-tag, you can detect the expression of this enzyme. Besides, you can purify the protein using Ni-colume and gel filtraion. As a result, you can build a system to product C6HSL in vitro. Between the His-tag and protein, we add the cutting site of thrombin. So that you can cut off the his-tag from the protein. false false _1018_ 0 8494 9 It's complicated false We want to find a way to detect the expression and purify LuxI protein. false Yu Zhao annotation2193941 1 Poly-hig tag range2193941 1 13 30 annotation2193942 1 LuxI without LVA tag range2193942 1 70 639 annotation2193943 1 thrombin cutting site range2193943 1 40 57 BBa_K766000_sequence 1 atgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z