BBa_K766005
1
BBa_K766005
T7 promoter-Hig tag-LuxI wihout LVA tag
2012-09-23T11:00:00Z
2015-05-08T01:13:14Z
We clone this gene from our lab's plamid which is commonly used
We build this part to work as the signal sender in our project. T7 promoter is a high-efficient promoter which can bind with T7 polymerase and start the transcription. Besides, we add the lac operator between T7 promoter and CDS.
If you use this part in a lacI-coding plasmid like pet15b, the expression of gene will be repressed. However, IPTG can be used to activate the expression. The expression of this part can be well-controled.
His tag is on the N-terminal, which consists of six histidine amino acid. We can use Ni-column to purify the protein. Besides, you can use Hig-tag antibody to detect the protein in western blot. This design makes the detection of luxI gene become very easy.
On the 5' end of sequence, we add Bgl2 cutting site. On the 5' end of luxI gene, we add Nde1 cuting site. On the 3'end of LuxI gene, we add Xho1 and BamH1 cutting site. All of this design is for the convenience of further use.
This part can be only used in the E.coli strains that can express T7 polymerase like BL21(DE3).
false
false
_1018_
0
8494
9
Not in stock
true
We want to build a signal sender which is easy detected and controled.
false
Yu Zhao
annotation2194873
1
T7 promoter
range2194873
1
20
37
annotation2194876
1
LuxI without LVA tag
range2194876
1
177
746
annotation2194875
1
Poly his tag
range2194875
1
120
137
annotation2194874
1
Lac operator
range2194874
1
39
65
annotation2194877
1
T7 terminator
range2194877
1
819
866
BBa_K766005_sequence
1
agatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaactcgaggatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z