LOCUS       BBa_K769003             2960 bp    DNA     linear       27-May-2024
DEFINITION  Pconst.(High)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-Double 
            terminator-PompR-RBS-GFP-Double terminator
ACCESSION   BBa_K769003
VERSION     BBa_K769003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23111
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1825
                     /label=BBa_K769004
     stem_loop       1845..1888
                     /label=stem_loop
     misc_feature    1834..1913
                     /label=BBa_B0010
     terminator      1834..1913
                     /label=BBa_B0010
     stem_loop       1929..1948
                     /label=T7 TE
     misc_feature    1955..1955
                     /label=stop
     polyA_site      1949..1962
                     /label=polya
     misc_feature    1922..1962
                     /label=BBa_B0012
     terminator      1922..1962
                     /label=BBa_B0012
     terminator      1834..1962
                     /label=BBa_B0015
     misc_feature    2091..2094
                     /label=conserved
     RBS             2087..2098
                     /label=BBa_B0034
     stem_loop       2843..2886
                     /label=stem_loop
     misc_feature    2832..2911
                     /label=BBa_B0010
     terminator      2832..2911
                     /label=BBa_B0010
     stem_loop       2927..2946
                     /label=T7 TE
     misc_feature    2953..2953
                     /label=stop
     polyA_site      2947..2960
                     /label=polya
     misc_feature    2920..2960
                     /label=BBa_B0012
     terminator      2920..2960
                     /label=BBa_B0012
     terminator      2832..2960
                     /label=BBa_B0015
     misc_feature    1971..2078
                     /label=BBa_R0082
     misc_feature    2087..2098
                     /label=BBa_B0034
     CDS             2107..2823
                     /label=GFP
     misc_feature    2832..2960
                     /label=BBa_B0015
     misc_feature    1971..2960
                     /label=BBa_K769001
ORIGIN
        1 ttgacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtggga cttacgaccc tcttttggct cggcgcaatc ggcatgctcg tcggcacgct
      121 cgcgttcgcg tgggccggcc gtgacgccgg aagcggcgag cgacggtact acgtgacact
      181 tgtcggcatc agtggtatcg cagcagtcgc ctacgccgtt atggcgctgg gtgtcggctg
      241 ggttcccgtg gccgaacgga ctgttttcgt cccccggtac atcgactgga ttctcacaac
      301 cccgctcatc gtctacttcc tcgggctgct tgcggggctt gatagtcggg agttcggcat
      361 cgtcatcacg ctcaacaccg tggtcatgct cgccggcttc gccggggcga tggtgcccgg
      421 tatcgagcgc tacgcgctgt tcggcatggg ggcggtcgca ttcatcggac tggtctacta
      481 cctcgtcggg ccgatgaccg aaagcgccag ccagcggtcc tccggaatca agtcgctgta
      541 cgtccgcctc cgaaacctga cggtcgtcct ctgggcgatt tatccgttca tctggctgct
      601 tggaccgccg ggcgtggcgc tgctgacacc gactgtcgac gtggcgctta tcgtctacct
      661 tgacctcgtc acgaaggtcg ggttcggctt catcgcactc gatgctgcgg cgacacttcg
      721 ggccgaacac ggcgcgtcgg cgtcgaacgg cgcgtcggcg atgtcgctga acgtatcacg
      781 gctccttctc cccggccgtg tccggcacag ttatacgggg aaaatgggtg ccaccttcgc
      841 ctttgtcggc gcgttaacgg tgctcttcgg agccatcgca tacggtgagg taaccgccgc
      901 cgccgcgacc ggcgatgccg cagccgtaca ggaggcggca gtatcggcca ttctcgggct
      961 catcatcctg ctcgggatca acctcgggct cgttgctgcc acgctgggcg gtgacaccgc
     1021 cgcctcgctt tcaacgctgg ccgcgaaggc ctcgcggatg ggcgacggcg acctcgatgt
     1081 cgagcttgag acccgtcgcg aggacgaaat cggcgacctc tatgcggcct tcgaccatat
     1141 ggcggctggt gttaagcaac tggcggatga ccgcacgctg ctgatggcgg gggtaagtca
     1201 cgacttgcgc acgccgctga cgcgtattcg cctggcgact gagatgatga gcgagcagga
     1261 tggctatctg gcagaatcga tcaataaaga tatcgaagag tgcaacgcca tcattgagca
     1321 gtttatcgac tacctgcgca ccgggcagga gatgccgatg gaaatggcgg atcttaatgc
     1381 agtactcggt gaggtgattg ctgccgaaag tggctatgag cgggaaattg aaaccgcgct
     1441 ttaccccggc agcattgaag tgaaaatgca cccgctgtcg atcaaacgcg cggtggcgaa
     1501 tatggtggtc aacgccgccc gttatggcaa tggctgggtc aaagtcagca gcggaacgga
     1561 gccgaatcgc gcctggttcc aggtggaaga tgacggtccg ggaattgcgc cggaacaacg
     1621 taagcacctg ttccagccgt ttgtccgcgg cgacagtgcg cgcaccatta gcggcacggg
     1681 attagggctg gcaattgtgc agcgtatcgt ggataaccat aacgggatgc tggagcttgg
     1741 caccagcgag cggggcgggc tttccattcg cgcctggctg ccagtgccgg taacgcgggc
     1801 gcagggcatg acaaaagaag ggtaatacta gagccaggca tcaaataaaa cgaaaggctc
     1861 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1921 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag tcccttgcat
     1981 ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta gtatcatatt
     2041 cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag aggagaaata
     2101 ctagagatga gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
     2161 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
     2221 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
     2281 acacttgtca ctactttctc ttatggtgtt caatgctttt cccgttatcc ggatcatatg
     2341 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga acgcactata
     2401 tctttcaaag atgacgggaa ctacaagacg cgtgctgaag tcaagtttga aggtgatacc
     2461 cttgttaatc gtatcgagtt aaaaggtatt gattttaaag aagatggaaa cattctcgga
     2521 cacaaactcg agtacaacta taactcacac aatgtataca tcacggcaga caaacaaaag
     2581 aatggaatca aagctaactt caaaattcgc cacaacattg aagatggatc cgttcaacta
     2641 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     2701 cattacctgt cgacacaatc tgccctttcg aaagatccca acgaaaagcg tgaccacatg
     2761 gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga gctctacaaa
     2821 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2881 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2941 tgggcctttc tgcgtttata
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