LOCUS       BBa_K772102             1059 bp    DNA     linear       07-May-2024
DEFINITION  Halorhodopsin ready-to-use system
ACCESSION   BBa_K772102
VERSION     BBa_K772102.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     promoter        229..244
                     /label=T7 promotor
     misc_feature    250..270
                     /label=lac repressor
     CDS             317..956
                     /label=Halorhodopsin
     RBS             294..305
                     /label=RBS
     CDS             229..956
                     /label=BBa_K559001
     stem_loop       972..991
                     /label=T7 TE
     misc_feature    998..998
                     /label=stop
     polyA_site      992..1005
                     /label=polya
     misc_feature    965..1005
                     /label=BBa_B0012
     terminator      965..1005
                     /label=BBa_B0012
     stem_loop       1026..1048
                     /label=stem_loop
     misc_feature    1014..1059
                     /label=BBa_B0011
     terminator      1014..1059
                     /label=BBa_B0011
     terminator      965..1059
                     /label=BBa_B0014
     misc_feature    229..1059
                     /label=BBa_K559010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagta atacgactca
      241 ctatagggga attgtgagcg gataacaatt ccccaataat tttgtttaac tttaagaaga
      301 agatatagta ctagagatga ctgagacatt gccaccggta acggaatcgg ctgttgcgct
      361 acaggcggag gtgacccaga gggagctgtt cgagttcgtt ctcaacgacc ccctcctcgc
      421 cagttcgctg tatattaata tcgcactggc agggctgtcg atactgcttt tcgtgttcat
      481 gacgcgcgga ctcgacgacc cacgggcgaa actcatcgcc gtttcgacga ttttggtgcc
      541 ggtggtctct atcgcgagct acaccggcct tgcatcgggg ctcaccatca gcgtcctcga
      601 gatgccagcc ggccacttcg ccgaggggtc ctcggtgatg ctcggcggcg aagaggtaga
      661 cggcgtcgtg acgatgtggg gccgctatct gacgtgggcc ctttcgacac cgatgatact
      721 gctggcgctt gggctgcttg ctggctctaa cgccacgaag ctctttaccg ccatcacctt
      781 cgacatcgcg atgtgtgtca ccggcctcgc agccgcgctg acgacctctt cgcacctgat
      841 gcggtggttc tggtacgcca tcagttgtgc gtgtttcctc gtcgtcctct acatcctgct
      901 cgtcgagtgg gcacaggacg ccaaggctgc cggtactgcg gatatgttca atacgctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat
     1021 ataaaaagcc agattattaa tccggctttt ttattattt
//