BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_K772102
1
BBa_K772102
Halorhodopsin ready-to-use system
2012-09-25T11:00:00Z
2015-05-08T01:13:15Z
Please refer to http://partsregistry.org/Part:BBa_K559000:Design, both of the genes are gathered from the Registry distribution.
Halorhodopsin is an inward-directed light-driven chloride ion pump originating from Halobacterium. It utilizes light to pump chloride ions against chloride concentration difference into cells from the environment.
We added a constitutive working and regulatable pLac promoter in the upstream region of the part in order to use it in our design easily.
false
false
_1024_
0
9573
9
Not in stock
false
Please refer to http://partsregistry.org/Part:BBa_K559000:Design for more detail about halorhodopsin.
Please refer to http://partsregistry.org/Part:BBa_J04500:Design for more detail about pLac promoter.
false
Mustafa Elitok
component2202534
1
BBa_K559010
component2202521
1
BBa_J04500
annotation2202534
1
BBa_K559010
range2202534
1
229
1059
annotation2202521
1
BBa_J04500
range2202521
1
1
220
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961227
1
start
range1961227
1
173
173
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939303
1
BBa_B0012
range939303
1
1
41
annotation939311
1
BBa_B0011
range939311
1
50
95
BBa_K559001
1
BBa_K559001
Halorhodopsin under T7 promoter
2011-09-28T11:00:00Z
2015-05-08T01:12:41Z
Halorhodopsin come from N.pharaonis, gene bank J05199.1
Halorhodopsin under constitute T7 promoter to regulate the gene transcription to be maximized and no restrict to other regulatory factor
false
false
_727_
0
6123
9
It's complicated
false
The coding sequence of N.pharaonis J05199.1 is selected from 61nt to 700nt and the T7 promotor placed in front by 3A assembly
false
Jacky, Fong Chuen, Loo
annotation2177551
1
T7 promotor
range2177551
1
1
16
annotation2177554
1
Halorhodopsin
range2177554
1
89
728
annotation2177553
1
lac repressor
range2177553
1
22
42
annotation2177555
1
RBS
range2177555
1
66
77
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K559010
1
BBa_K559010
Halorhodopsin complete system
2011-09-28T11:00:00Z
2015-05-08T01:12:41Z
Halorhodopsin comes from part BBa_K559000, which is come from genomic sequence of N.pharaonis (genebank ID:J05199.1)
Halorhodopsin gene allow chloride ion to be pumped in by the level of light intensity. Halorhodopsin gene is under regulation by constitute T7 promoter to allow protein coding to be maximized, and the bidirectional terminator is added for high efficiency termination of both forward and backward transcription
false
false
_727_
0
6123
9
It's complicated
true
The double terminator wass tested to stop effectively on the transcription of both forward and backward transcription to make our protein generator be a controlled system
false
Jacky, Fong Chuen, Loo
component2177574
1
BBa_K559001
component2177581
1
BBa_B0014
annotation2177574
1
BBa_K559001
range2177574
1
1
728
annotation2177581
1
BBa_B0014
range2177581
1
737
831
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508159
1
BBa_B0034
component1508149
1
BBa_R0010
annotation1508149
1
BBa_R0010
range1508149
1
1
200
annotation1508159
1
BBa_B0034
range1508159
1
209
220
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K559001_sequence
1
taatacgactcactataggggaattgtgagcggataacaattccccaataattttgtttaactttaagaagaagatatagtactagagatgactgagacattgccaccggtaacggaatcggctgttgcgctacaggcggaggtgacccagagggagctgttcgagttcgttctcaacgaccccctcctcgccagttcgctgtatattaatatcgcactggcagggctgtcgatactgcttttcgtgttcatgacgcgcggactcgacgacccacgggcgaaactcatcgccgtttcgacgattttggtgccggtggtctctatcgcgagctacaccggccttgcatcggggctcaccatcagcgtcctcgagatgccagccggccacttcgccgaggggtcctcggtgatgctcggcggcgaagaggtagacggcgtcgtgacgatgtggggccgctatctgacgtgggccctttcgacaccgatgatactgctggcgcttgggctgcttgctggctctaacgccacgaagctctttaccgccatcaccttcgacatcgcgatgtgtgtcaccggcctcgcagccgcgctgacgacctcttcgcacctgatgcggtggttctggtacgccatcagttgtgcgtgtttcctcgtcgtcctctacatcctgctcgtcgagtgggcacaggacgccaaggctgccggtactgcggatatgttcaatacgc
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_K772102_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagtaatacgactcactataggggaattgtgagcggataacaattccccaataattttgtttaactttaagaagaagatatagtactagagatgactgagacattgccaccggtaacggaatcggctgttgcgctacaggcggaggtgacccagagggagctgttcgagttcgttctcaacgaccccctcctcgccagttcgctgtatattaatatcgcactggcagggctgtcgatactgcttttcgtgttcatgacgcgcggactcgacgacccacgggcgaaactcatcgccgtttcgacgattttggtgccggtggtctctatcgcgagctacaccggccttgcatcggggctcaccatcagcgtcctcgagatgccagccggccacttcgccgaggggtcctcggtgatgctcggcggcgaagaggtagacggcgtcgtgacgatgtggggccgctatctgacgtgggccctttcgacaccgatgatactgctggcgcttgggctgcttgctggctctaacgccacgaagctctttaccgccatcaccttcgacatcgcgatgtgtgtcaccggcctcgcagccgcgctgacgacctcttcgcacctgatgcggtggttctggtacgccatcagttgtgcgtgtttcctcgtcgtcctctacatcctgctcgtcgagtgggcacaggacgccaaggctgccggtactgcggatatgttcaatacgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K559010_sequence
1
taatacgactcactataggggaattgtgagcggataacaattccccaataattttgtttaactttaagaagaagatatagtactagagatgactgagacattgccaccggtaacggaatcggctgttgcgctacaggcggaggtgacccagagggagctgttcgagttcgttctcaacgaccccctcctcgccagttcgctgtatattaatatcgcactggcagggctgtcgatactgcttttcgtgttcatgacgcgcggactcgacgacccacgggcgaaactcatcgccgtttcgacgattttggtgccggtggtctctatcgcgagctacaccggccttgcatcggggctcaccatcagcgtcctcgagatgccagccggccacttcgccgaggggtcctcggtgatgctcggcggcgaagaggtagacggcgtcgtgacgatgtggggccgctatctgacgtgggccctttcgacaccgatgatactgctggcgcttgggctgcttgctggctctaacgccacgaagctctttaccgccatcaccttcgacatcgcgatgtgtgtcaccggcctcgcagccgcgctgacgacctcttcgcacctgatgcggtggttctggtacgccatcagttgtgcgtgtttcctcgtcgtcctctacatcctgctcgtcgagtgggcacaggacgccaaggctgccggtactgcggatatgttcaatacgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z