LOCUS       BBa_K775000             1414 bp    DNA     linear       17-May-2024
DEFINITION  RI7-GPR109A Niacin chimeric GPCR
ACCESSION   BBa_K775000
VERSION     BBa_K775000.1  
FEATURES             Location/Qualifiers
     promoter        1..112
                     /label=GPD promoter
     misc_feature    113..118
                     /label=BamHI restriction site
     RBS             119..124
                     /label=Kozak sequence
     misc_feature    125..127
                     /label=Startcodon
     misc_feature    128..151
                     /label=FLAGtag
     misc_feature    152..337
                     /label=N terminus RI7
     misc_feature    1151..1156
                     /label=Stopcodon
     misc_feature    1157..1162
                     /label=NdeI restriction site
     misc_feature    1163..1414
                     /label=CYC1 terminator
     misc_feature    1055..1150
                     /label=C terminus RI7
     CDS             338..1054
                     /label=Niacin receptor part
ORIGIN
        1 cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt
       61 atagttagtc ttttttttag ttttaaaaca ccagaactta gtttcgacgg atggatccaa
      121 aaaaatggac tacaaggacg acgacgacaa ggagcgaagg aaccacagtg ggagagtgag
      181 tgaatttgtg ttgctgggtt tcccagctcc tgccccactg cgagtactac tatttttcct
      241 ttctcttctg gcctatgtgt tggtgttgac tgaaaacatg ctcatcatta tagcaattag
      301 gaaccaccca accctccaca aacccatgta tcatctcaaa tcctggaaat ccagccggat
      361 tttcttgttc aacctggccg tggctgactt tctcctgatc atttgcttgc cgttcttgac
      421 ggacaactat gtccagaact gggactggag gttcgggagc atcccctgcc gcgtgatgct
      481 cttcatgttg gccatgaacc gacagggcag catcatcttc ctcacggtgg tggctgtgga
      541 caggtacttc agggtggtcc acccgcacca cttcctgaac aagatctcca accggacggc
      601 ggccatcatc tcttgcttcc tgtggggcat caccatcggc ctgacagtcc acctcctcta
      661 cacggacatg atgacccgaa acggcgatgc aaacctgtgc agcagtttta gcatctgcta
      721 cactttcagg tggcacgatg caatgttcct cttggagttc ttcctgcccc tgggcatcat
      781 cctgttctgc tctggcagga tcatttggag cctaaggcag agacagatgg acaggcacgt
      841 caagatcaag agggccatca acttcatcat ggtggttgcc attgtgtttg tcatctgctt
      901 cctgcccagt gtggccgtga ggacccgcat cttctggctc ctctacaaac acaacgtgag
      961 gaactgtgac atctactcct ctgtggactt ggccttcttc accaccctta gctttaccta
     1021 catgaacagc atgctcgacc cggtggtcta ctactgcttg cgcaaccaag atgtcaaaag
     1081 agcgctacgt cgcacgctgc acctggccca ggaccaggag gccaatacca acaaaggcag
     1141 caaaattggt taataacata tgtcatgtaa ttagttatgt cacgcttaca ttcacgccct
     1201 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     1261 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     1321 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     1381 gttttgggac gctcgaaggc tttaatttgc ggcc
//