LOCUS       BBa_K775009             2588 bp    DNA     linear       10-May-2024
DEFINITION  Niacin Chimeric GPCR with Fus1-EGFP reporter (2)
ACCESSION   BBa_K775009
VERSION     BBa_K775009.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=FUS1 promoter
     misc_feature    201..206
                     /label=BamHi restriction site
     RBS             207..212
                     /label=Kozak sequence
     CDS             213..936
                     /label=EGFP
     misc_feature    937..942
                     /label=NdeI restriction site
     misc_feature    942..1166
                     /label=ADH1 terminator
     misc_feature    1..1166
                     /label=BBa_K775004
     promoter        1175..1286
                     /label=GPD promoter
     misc_feature    1287..1292
                     /label=BamHI restriction site
     RBS             1293..1298
                     /label=Kozak sequence
     misc_feature    1299..1301
                     /label=Startcodon
     misc_feature    1302..1325
                     /label=FLAGtag
     misc_feature    1326..1511
                     /label=N terminus RI7
     misc_feature    2325..2330
                     /label=Stopcodon
     misc_feature    2331..2336
                     /label=NdeI restriction site
     misc_feature    2337..2588
                     /label=CYC1 terminator
     misc_feature    2229..2324
                     /label=C terminus RI7
     CDS             1512..2228
                     /label=Niacin receptor part
     CDS             1175..2588
                     /label=BBa_K775000
ORIGIN
        1 ttttggtgcg atgatgaaac aaacatgaaa cgtcagtaat ttgaaacaaa taacgtaatt
       61 ctcgggattg gttttattta aatgacaatg taagagtggc tttgtaaggt atgtgttgct
      121 cttaaaatat ttggatacga catcctttat ctttttcctt taagagcagg atataagcca
      181 tcaagtttct gaaaatcaaa ggatccaaaa aaatggtgag caagggcgag gagctgttca
      241 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      301 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      361 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      421 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      481 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      541 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      601 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      661 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      721 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      781 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
      841 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      901 tcactctcgg catggacgag ctgtacaagt aataacatat gtaataagcg aatttcttat
      961 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1021 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     1081 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     1141 cgagcaaatg cctgcaaatc gctccctact agagcggtag gtattgattg taattctgta
     1201 aatctatttc ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa
     1261 aacaccagaa cttagtttcg acggatggat ccaaaaaaat ggactacaag gacgacgacg
     1321 acaaggagcg aaggaaccac agtgggagag tgagtgaatt tgtgttgctg ggtttcccag
     1381 ctcctgcccc actgcgagta ctactatttt tcctttctct tctggcctat gtgttggtgt
     1441 tgactgaaaa catgctcatc attatagcaa ttaggaacca cccaaccctc cacaaaccca
     1501 tgtatcatct caaatcctgg aaatccagcc ggattttctt gttcaacctg gccgtggctg
     1561 actttctcct gatcatttgc ttgccgttct tgacggacaa ctatgtccag aactgggact
     1621 ggaggttcgg gagcatcccc tgccgcgtga tgctcttcat gttggccatg aaccgacagg
     1681 gcagcatcat cttcctcacg gtggtggctg tggacaggta cttcagggtg gtccacccgc
     1741 accacttcct gaacaagatc tccaaccgga cggcggccat catctcttgc ttcctgtggg
     1801 gcatcaccat cggcctgaca gtccacctcc tctacacgga catgatgacc cgaaacggcg
     1861 atgcaaacct gtgcagcagt tttagcatct gctacacttt caggtggcac gatgcaatgt
     1921 tcctcttgga gttcttcctg cccctgggca tcatcctgtt ctgctctggc aggatcattt
     1981 ggagcctaag gcagagacag atggacaggc acgtcaagat caagagggcc atcaacttca
     2041 tcatggtggt tgccattgtg tttgtcatct gcttcctgcc cagtgtggcc gtgaggaccc
     2101 gcatcttctg gctcctctac aaacacaacg tgaggaactg tgacatctac tcctctgtgg
     2161 acttggcctt cttcaccacc cttagcttta cctacatgaa cagcatgctc gacccggtgg
     2221 tctactactg cttgcgcaac caagatgtca aaagagcgct acgtcgcacg ctgcacctgg
     2281 cccaggacca ggaggccaat accaacaaag gcagcaaaat tggttaataa catatgtcat
     2341 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
     2401 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
     2461 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
     2521 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
     2581 ttgcggcc
//