BBa_K777121

BBa_K777121 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K777121

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Flash Coli

Date created: 2012-09-17 11:00:00

Date modified: 2015-05-08 01:13:17

yhjH

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K777121_sequence (Version 1)

Description

The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in yhjH-mutants.

Notes

• yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
  • Fwd: 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATAAGGCAGGTTATCCAGC-3'
  • Rev: 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATAGCGCCAGAACCGCCGTATTCAGC-3'

Source

The part was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
YhjH coding sequence	1,768	feature/cds, CDS
start	1,1	start_codon, feature/start
stop	768,768	stop_codon, feature/stop

Other Properties

• igem#experience: Works
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• rdf-schema#seeAlso: P37646
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K777121/1

Member of these Collections

• //cds/enzyme
• //chassis/prokaryote/ecoli
• //function/motility
• iGEM Parts Registry