BBa_K777123

BBa_K777123 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K777123
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Goettingen
Date created: 2012-09-21 11:00:00
Date modified: 2015-05-08 01:13:17

yhjH under the control of constitutive promoter J23112



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K777123_sequence (Version 1)

Description

The product of this gene is a phosphodiesterase which is involved in regulating the levels of c-di-GMP, a bacterial signaling molecule. High levels of c-di-GMP promote biofilm synthesis and inhibit flagella by binding to YcgR which then acts as a "brake" on FliG and FliM. Levels of c-di-GMP are elevated and motility is reduced in yhjH-mutants.

Here we used 3 different constitutive Anderson promoters from the 2006 Berkeley group to test how different levels of constitutive yhjH expression affect the motility of E. coli.

Notes

  • yhjH was amplified from genomic DNA of E. coli DH10B via PCR using the following primers:
    • Fwd: 5'-gtttcttcgaattcgcggccgcttctagatgataaggcaggttatccagc-3'
    • Rev: 5'-gtttcttcctgcagcggccgctactagtattatagcgccagaaccgccgtattcagc-3'


Source

  • yhjH was amplified from genomic DNA of E. coli str. K-12 substr. DH10B, complete genome (CP000948.1).
  • J23100 information was taken from the partsregistry and physical DNA from the 2012 distribution kit.


igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K777123/1